Anti-PKA I alpha reg PRKAR1A Antibody

Boster Bio Anti-PKA I alpha reg PRKAR1A Antibody catalog # A00699-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00699-2
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-PKA I alpha reg PRKAR1A Antibody

See all Protein Kinase A regulatory subunit I alpha products

SKU/Catalog Number

A00699-2

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PKA I alpha reg PRKAR1A Antibody catalog # A00699-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PKA I alpha reg PRKAR1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00699-2)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Reactive Species

A00699-2 is reactive to PRKAR1A in Human, Mouse, Rat

Applications

A00699-2 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PRKAR1A (Source: Uniprot.Org, NCBI)

Uniprot ID

P10644

Gene ID

5573

Gene Name

PRKAR1A

Full Name

cAMP-dependent protein kinase type I-alpha regulatory subunit

Weight

42.982kDa

Superfamily

cAMP-dependent kinase regulatory chain family

Alternative Names

cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; CAR; CNC; CNC1; MGC17251; PKA R1 alpha; PKA1; PKR1; PPNAD1; PRKAR1; protein kinase A type 1a regulatory subunit; protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specificextinguisher 1); Tissue-specific extinguisher 1; TSE1DKFZp779L0468 PRKAR1A ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, TSE1 protein kinase cAMP-dependent type I regulatory subunit alpha cAMP-dependent protein kinase type I-alpha regulatory subunit|Carney complex type 1|cAMP-dependent protein kinase regulatory subunit RIalpha|cAMP-dependent protein kinase type I-alpha regulatory chain|epididymis secretory sperm binding protein|protein kinase A type 1a regulatory subunit|protein kinase, cAMP-dependent, regulatory subunit type I alpha|protein kinase, cAMP-dependent, regulatory, type I, alpha|tissue-specific extinguisher 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PRKAR1A, check out the PRKAR1A Infographic

PRKAR1A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PRKAR1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-PKA I alpha reg PRKAR1A Antibody

Question

My question regarding product A00699-2, anti-PKA I alpha reg antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00699-2 anti-PKA I alpha reg antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-20

Question

See below the WB image, lot number and protocol we used for platelet using anti-PKA I alpha reg antibody A00699-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-18

Question

Will anti-PKA I alpha reg antibody A00699-2 work for WB with platelet?

K. Singh

Verified customer

Asked: 2017-11-29

Answer

According to the expression profile of platelet, PRKAR1A is highly expressed in platelet. So, it is likely that anti-PKA I alpha reg antibody A00699-2 will work for WB with platelet.

Boster Scientific Support

Answered: 2017-11-29

Question

Is a blocking peptide available for product anti-PKA I alpha reg antibody (A00699-2)?

Verified Customer

Verified customer

Asked: 2017-08-11

Answer

We do provide the blocking peptide for product anti-PKA I alpha reg antibody (A00699-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-08-11

Question

Would A00699-2 anti-PKA I alpha reg antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

It shows on the product datasheet, A00699-2 anti-PKA I alpha reg antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-07-10

Question

I see that the anti-PKA I alpha reg antibody A00699-2 works with WB, what is the protocol used to produce the result images on the product page?

Z. Huang

Verified customer

Asked: 2015-03-27

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-03-27

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00699-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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