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Product Info Summary
| SKU: | A00699-2 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-PKA I alpha reg PRKAR1A Antibody
SKU/Catalog Number
A00699-2
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PKA I alpha reg PRKAR1A Antibody catalog # A00699-2. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKA I alpha reg PRKAR1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00699-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human KAP0. AA range:271-320
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00699-2 is reactive to PRKAR1A in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00699-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of 293 cells using PKA Iα reg Polyclonal Antibody
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Western blot analysis of lysates from HepG2 cells, using KAP0 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-PKA I alpha reg PRKAR1A Antibody (A00699-2)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PKA I alpha reg PRKAR1A Antibody
Question
My question regarding product A00699-2, anti-PKA I alpha reg antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00699-2 anti-PKA I alpha reg antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-20
Question
See below the WB image, lot number and protocol we used for platelet using anti-PKA I alpha reg antibody A00699-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-18
Question
Will anti-PKA I alpha reg antibody A00699-2 work for WB with platelet?
K. Singh
Verified customer
Asked: 2017-11-29
Answer
According to the expression profile of platelet, PRKAR1A is highly expressed in platelet. So, it is likely that anti-PKA I alpha reg antibody A00699-2 will work for WB with platelet.
Boster Scientific Support
Answered: 2017-11-29
Question
Is a blocking peptide available for product anti-PKA I alpha reg antibody (A00699-2)?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
We do provide the blocking peptide for product anti-PKA I alpha reg antibody (A00699-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-08-11
Question
Would A00699-2 anti-PKA I alpha reg antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
It shows on the product datasheet, A00699-2 anti-PKA I alpha reg antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-10
Question
I see that the anti-PKA I alpha reg antibody A00699-2 works with WB, what is the protocol used to produce the result images on the product page?
Z. Huang
Verified customer
Asked: 2015-03-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-03-27
