Anti-DARS2 Antibody Picoband™

DARS2 antibody

Boster Bio Anti-DARS2 Antibody Picoband™ catalog # A06034-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A06034-1
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-DARS2 Antibody Picoband™

View all DARS2 Antibodies

SKU/Catalog Number

A06034-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DARS2 Antibody Picoband™ catalog # A06034-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DARS2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06034-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human DARS2 recombinant protein (Position: D334-A448).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A06034-1 is reactive to DARS2 in Human

Applications

A06034-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Background of DARS2

DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). The International Radiation Hybrid Mapping Consortium mapped the DARS2 gene to chromosome 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For DARS2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q6PI48

Gene ID

55157

Gene Name

DARS2

Full Name

Aspartate--tRNA ligase, mitochondrial

Weight

73.563kDa

Superfamily

class-II aminoacyl-tRNA synthetase family

Alternative Names

aspartate tRNA ligase 2, mitochondrial; Aspartate--tRNA ligase; aspartyl-tRNA synthetase 2, mitochondrial; aspartyl-tRNA synthetase, mitochondrial; AspRS; ASPRS. LBSL; EC 6.1.1; EC 6.1.1.12; FLJ10514; LBSL; MT-ASPRS; RP3-383J4.2 DARS2 ASPRS, LBSL, MT-ASPRS, mtAspRS aspartyl-tRNA synthetase 2, mitochondrial aspartate--tRNA ligase, mitochondrial|aspartate tRNA ligase 2, mitochondrial|aspartyl-tRNA synthetase, mitochondrial

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DARS2, check out the DARS2 Infographic

DARS2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DARS2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-DARS2 Antibody Picoband™

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-DARS2 antibody A06034-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-09

Question

Would A06034-1 anti-DARS2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

As indicated on the product datasheet, A06034-1 anti-DARS2 antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-01

Question

My question regards to test anti-DARS2 antibody A06034-1 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-DARS2 antibody A06034-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The products we sell, including anti-DARS2 antibody A06034-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-15

Question

I have a question about product A06034-1, anti-DARS2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06034-1 anti-DARS2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-28

Question

Has A06034-1 been tested on mouse and rat samples?

Verified customer

Asked: 2019-04-24

Answer

For Anti-DARS2 Antibody Picoband™ (A06034-1), it did not test well for mouse and rat samples. We do not recommend this antibody on mouse and rat samples.

Boster Scientific Support

Answered: 2019-04-24

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-DARS2 antibody A06034-1. Let me know if you need anything else.

D. Mangal

Verified customer

Asked: 2015-12-30

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-12-30

Question

We are currently using anti-DARS2 antibody A06034-1 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?

A. Carter

Verified customer

Asked: 2015-02-06

Answer

The anti-DARS2 antibody (A06034-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-02-06

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A06034-1

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A06034-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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