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Product Info Summary
| SKU: | M03817 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-GNA11 Antibody (Center)
SKU/Catalog Number
M03817
Size
80 µl
Description
Boster Bio Anti-GNA11 Antibody (Center) (Catalog # M03817). Tested in WB, IF application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GNA11 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03817)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GNA11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 115-146 amino acids from the Central region of human GNA11.
Reactive Species
M03817 is reactive to GNA11 in Human, Mouse
Calculated molecular weight
42.1 kDa
Background of GNA11
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03817 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling GNA11 with M03817 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and membrane staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).
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All lanes : Anti-GNA11 Antibody (Center) at 1:2000 dilution
Lane 1: human testis lysates
Lane 2: Hela whole cell lysates
Lane 3: NIH/3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 36 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-GNA11 Antibody (Center) (M03817)
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