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Product Info Summary
| SKU: | M00743 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PKC alpha PRKCA Rabbit Monoclonal Antibody
SKU/Catalog Number
M00743
BM4238 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PKC alpha PRKCA Rabbit Monoclonal Antibody catalog # M00743. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC alpha PRKCA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00743)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CHO-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PKC alpha
Reactive Species
M00743 is reactive to PRKCA in Human, Mouse, Rat
Observed Molecular Weight
77 kDa
Calculated molecular weight
76.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00743 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, human A549 whole cell, rat liver tissue, rat spleen tissue, mouse liver tissue
IHC: Human pituitary tumor tissue, Human melanoma tissue, Human thymoma tissue, human colon tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:50 dilution.
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Western blot analysis of PRKCA using anti-PRKCA antibody (M00743).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCA antigen affinity purified monoclonal antibody (Catalog # M00743) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCA at approximately 77 kDa. The expected band size for PRKCA is at 77 kDa.
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Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human thymoma, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded human colon, using PKC alpha Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of Hela cells, using PKC alpha Antibody .
Specific Publications For Anti-PKC alpha PRKCA Rabbit Monoclonal Antibody (M00743)
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