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Product Info Summary
| SKU: | PB10021 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Abhd5 Antibody Picoband®
SKU/Catalog Number
PB10021
Size
100 μg/vial
Form
Lyophilized
Description
ABHD5 (CGI-58) regulates cellular triacylglycerol storage by activating PNPLA2/ATGL-dependent lipolysis at lipid droplets, linking it to intracellular lipid mobilization programs. Assay context (per provided product description; product page not retrievable via tool at curation time): Human/Rat-reactive Picoband antibody validated for IF/ICC, IHC, and WB to localize lipid-droplet–associated pools. Often interpreted with fatty-acid utilization and lipid routing markers such as ACSL5 and ANGPTL3 (putative co-panel spanning intracellular vs circulating lipid regulation).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Abhd5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10021)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Abhd5 recombinant protein (Position: R169-D349). Human Abhd5 shares 96.7% amino acid (aa) sequence identity with both mouse and rat Abhd5.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10021 is reactive to ABHD5 in Human, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
39.1 kDa
Background of ABHD5
1-acylglycerol-3-phosphate O-acyltransferase ABHD5 is an enzyme that in humans is encoded by the ABHD5 gene. The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated withChanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10021 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat kidney tissue, A431 whole cell
IHC: human intestinal cancer tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of Abhd5 using anti-Abhd5 antibody (PB10021).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: A431 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Abhd5 antigen affinity purified polyclonal antibody (Catalog # PB10021) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Abhd5 at approximately 43KD. The expected band size for Abhd5 is at 39KD.
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IHC analysis of Abhd5 using anti-Abhd5 antibody (PB10021).
Abhd5 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Abhd5 Antibody (PB10021) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IF analysis of Abhd5 using anti-Abhd5 antibody (PB10021).
Abhd5 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Abhd5 Antibody ((PB10021) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Abhd5 Antibody Picoband® (PB10021)
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1 Customer Q&As for Anti-Abhd5 Antibody Picoband®
Question
We are currently using anti-Abhd5 antibody PB10021 for rat tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on dog tissues as well?
M. Walker
Verified customer
Asked: 2019-09-13
Answer
The anti-Abhd5 antibody (PB10021) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-13
