Click image to see more details
Product Info Summary
| SKU: | M02271 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Vitronectin VTN Rabbit Monoclonal Antibody
SKU/Catalog Number
M02271
BM4292 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Vitronectin VTN Rabbit Monoclonal Antibody catalog # M02271. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vitronectin VTN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02271)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DCC-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Vitronectin
Reactive Species
M02271 is reactive to VTN in Human, Mouse, Rat
Observed Molecular Weight
75 kDa
Calculated molecular weight
54.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02271 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human Serum membrane
Validation Images & Assay Conditions
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Western blot analysis of Vitronectin expression in human Serum membrane lysate.
Specific Publications For Anti-Vitronectin VTN Rabbit Monoclonal Antibody (M02271)
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Customer Q&As
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4 Customer Q&As for Anti-Vitronectin VTN Rabbit Monoclonal Antibody
Question
Would anti-Vitronectin Rabbit Monoclonal antibody M02271 work on horse IF with platelet?
A. Dhar
Verified customer
Asked: 2019-10-30
Answer
Our lab technicians have not validated anti-Vitronectin Rabbit Monoclonal antibody M02271 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Vitronectin Rabbit Monoclonal antibody M02271 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse platelet in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-30
Question
Does anti-Vitronectin Rabbit Monoclonal antibody M02271 work for WB with mammary gland?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
According to the expression profile of mammary gland, VTN is highly expressed in mammary gland. So, it is likely that anti-Vitronectin Rabbit Monoclonal antibody M02271 will work for WB with mammary gland.
Boster Scientific Support
Answered: 2018-08-20
Question
I was wanting to use your anti-Vitronectin Rabbit Monoclonal antibody for WB for rat mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat mammary gland identification?
C. Parker
Verified customer
Asked: 2018-06-15
Answer
As indicated on the product datasheet, M02271 anti-Vitronectin Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-15
Question
See attached the WB image, lot number and protocol we used for mammary gland using anti-Vitronectin Rabbit Monoclonal antibody M02271. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-14
