Product Info Summary
SKU: | M01151-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI2A12]
View all PKC epsilon Antibodies
SKU/Catalog Number
M01151-1
Size
100 µl
Description
Boster Bio PRKCE (PKC epsilon ) mouse monoclonal antibody, clone OTI2A12 (formerly 2A12). Catalog# M01151-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI2A12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01151-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI2A12
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01151-1 is reactive to PRKCE in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
83.674kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01151-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells transfected with either PRKCE (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKCE antibody (M01151-1)
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Anti-PRKCE mouse monoclonal antibody (M01151-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKCE.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKCE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCE.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PRKCE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Protein Target Info & Infographic
Gene/Protein Information For PRKCE (Source: Uniprot.org, NCBI)
Gene Name
PRKCE
Full Name
Protein kinase C epsilon type
Weight
83.674kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.13; MGC125656; nPKC-epsilon; PKC epsilon; PKCE; PKCEMGC125657; PRKCE; protein kinase C epsilon type; protein kinase C, epsilon PRKCE PKCE, nPKC-epsilon protein kinase C epsilon protein kinase C epsilon type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCE, check out the PRKCE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI2A12] (M01151-1)
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