Click image to see more details
-
-
-
-
-
+10
Product Info Summary
| SKU: | M01151-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI2A12]
SKU/Catalog Number
M01151-1
Size
100 µl
Description
Boster Bio PRKCE (PKC epsilon ) mouse monoclonal antibody, clone OTI2A12 (formerly 2A12). Catalog# M01151-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI2A12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01151-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2A12
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.
Reactive Species
M01151-1 is reactive to PRKCE in Dog, Human, Monkey, Mouse, Rat
Calculated molecular weight
83.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01151-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000~2000
IHC 1:150
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
Click image to see more details
HEK293T cells transfected with either PRKCE (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKCE antibody (M01151-1)
Click image to see more details
Anti-PRKCE mouse monoclonal antibody (M01151-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKCE.
Click image to see more details
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKCE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCE.
Click image to see more details
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PRKCE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Specific Publications For Anti-PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI2A12] (M01151-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI2A12]?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI2A12]
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
