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Product Info Summary
| SKU: | A01999-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-EPHX2 Antibody Picoband®
SKU/Catalog Number
A01999-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPHX2 Antibody Picoband® catalog # A01999-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPHX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01999-1)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human EPHX2, different from the related mouse sequence by seven amino acids, and from the related rat sequence by eight amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01999-1 is reactive to EPHX2 in Human, Mouse, Rat
Observed Molecular Weight
63 kDa
Calculated molecular weight
62.6 kDa
Background of EPHX2
Soluble epoxide hydrolase (sEH) is a bifunctional enzyme that in humans is encoded by the EPHX2 gene. It is mapped to 8p21.2-p21.1. This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01999-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human HepG2 whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue
FCM: PC-3 cell
Validation Images & Assay Conditions
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Western blot analysis of EPHX2 using anti-EPHX2 antibody (A01999-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHX2 antigen affinity purified polyclonal antibody (Catalog # A01999-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHX2 at approximately 63 kDa. The expected band size for EPHX2 is at 63 kDa.
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Flow Cytometry analysis of PC-3 cells using anti-EPHX2 antibody (A01999-1).
Overlay histogram showing PC-3 cells stained with A01999-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPHX2 Antibody (A01999-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-EPHX2 Antibody Picoband® (A01999-1)
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Customer Q&As
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4 Customer Q&As for Anti-EPHX2 Antibody Picoband®
Question
Does anti-EPHX2 antibody A01999-1 work for Flow Cytometry with placenta?
J. Krishna
Verified customer
Asked: 2019-11-21
Answer
According to the expression profile of placenta, EPHX2 is highly expressed in placenta. So, it is likely that anti-EPHX2 antibody A01999-1 will work for Flow Cytometry with placenta.
Boster Scientific Support
Answered: 2019-11-21
Question
We need to test anti-EPHX2 antibody A01999-1 on mouse placenta for research purposes, then I may be interested in using anti-EPHX2 antibody A01999-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The products we sell, including anti-EPHX2 antibody A01999-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-EPHX2 antibody A01999-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The anti-EPHX2 antibody (A01999-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-16
Question
Does anti-EPHX2 antibody A01999-1 work on primate WB with right lobe of liver?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
Our lab technicians have not tested anti-EPHX2 antibody A01999-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-EPHX2 antibody A01999-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate right lobe of liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-07
