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Product Info Summary
| SKU: | A00583-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-Polycomb protein SUZ12 SUZ12 Antibody
SKU/Catalog Number
A00583-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Polycomb protein SUZ12 SUZ12 Antibody catalog # A00583-2. Tested in WB,ICC/IF,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Polycomb protein SUZ12 SUZ12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00583-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human SUZ12(NP_056170.2).
Reactive Species
A00583-2 is reactive to SUZ12 in Human, Mouse, Rat
Calculated molecular weight
83.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00583-2 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:100
IP: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using SUZ12 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
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Western blot analysis of extracts from normal and SUZ12 knockout HeLa cells, using SUZ12 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
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Immunofluorescence analysis of C6 cells using SUZ12 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SUZ12 antibody . Western blot was performed from the immunoprecipitate using SUZ12 antibody at a dilution of 1:500.
Specific Publications For Anti-Polycomb protein SUZ12 SUZ12 Antibody (A00583-2)
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