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Product Info Summary
| SKU: | A00583 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Polycomb protein SUZ12 SUZ12 Antibody
SKU/Catalog Number
A00583
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Polycomb protein SUZ12 SUZ12 Antibody (Catalog # A00583). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
SUZ12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Polycomb protein SUZ12 SUZ12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00583)
Host
Rabbit
Contents
SUZ12 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SUZ12 antibody was raised against a 19 amino acid peptide near the center of human SUZ12. The immunogen is located within amino acids 400 - 450 of SUZ12.
Cross-reactivity
SUZ12 antibody is human specific.
Reactive Species
A00583 is reactive to SUZ12 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
83.1 kDa
Background of SUZ12
The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis (1). SUZ12 (suppressor of zeste 12) is a 90 - 95 kDa member of the polycomb group of transcriptional regulators (2). It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. It contains one C2H2-type zinc finger region and a C-terminal VEFS-box (3,4). SUZ12 is overexpressed in breast and colon cancer. SUZ12 may be a cause of endometrial stromal tumors (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00583 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SUZ12 antibody can be used for detection of SUZ12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SUZ12 in human liver tissue lysate with SUZ12 antibody at 1 μg/ml.
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Immunohistochemistry of SUZ12 in human lung tissue with SUZ12 antibody at 5 μg/mL.
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Immunofluorescence of SUZ12 in human lung tissue with SUZ12 antibody at 20 μg/mL.
Specific Publications For Anti-Polycomb protein SUZ12 SUZ12 Antibody (A00583)
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