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Product Info Summary
| SKU: | A03027-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FBXL11/KDM2A Antibody Picoband®
SKU/Catalog Number
A03027-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FBXL11/KDM2A Antibody Picoband® catalog # A03027-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FBXL11/KDM2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03027-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FBXL11, which shares 97.1% and 100% amino acid (aa) sequence identity with mouse and rat FBXL11, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03027-1 is reactive to KDM2A in Human, Mouse, Rat
Observed Molecular Weight
150 kDa
Calculated molecular weight
132.8 kDa
Background of KDM2A
Lysine-specific demethylase 2A (KDM2A) also known as F-box and leucine-rich repeat protein 11 (FBXL11) is an enzyme that in humans is encoded by the KDM2A gene. This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03027-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: mouse liver tissue, human A431 cell
Validation Images & Assay Conditions
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Western blot analysis of FBXL11 using anti-FBXL11 antibody (A03027-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: human A431 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXL11 antigen affinity purified polyclonal antibody (Catalog # A03027-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FBXL11 at approximately 150KD. The expected band size for FBXL11 is at 133KD.
Specific Publications For Anti-FBXL11/KDM2A Antibody Picoband® (A03027-1)
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Customer Q&As
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7 Customer Q&As for Anti-FBXL11/KDM2A Antibody Picoband®
Question
Is a blocking peptide available for product anti-FBXL11/KDM2A antibody (A03027-1)?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
We do provide the blocking peptide for product anti-FBXL11/KDM2A antibody (A03027-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-27
Question
I see that the anti-FBXL11/KDM2A antibody A03027-1 works with WB, what is the protocol used to produce the result images on the product page?
C. Krishna
Verified customer
Asked: 2019-09-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-17
Question
Here is the WB image, lot number and protocol we used for spleen using anti-FBXL11/KDM2A antibody A03027-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-15
Question
Would A03027-1 anti-FBXL11/KDM2A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
It shows on the product datasheet, A03027-1 anti-FBXL11/KDM2A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-11
Question
My question regarding product A03027-1, anti-FBXL11/KDM2A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03027-1 anti-FBXL11/KDM2A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-23
Question
I am interested in to test anti-FBXL11/KDM2A antibody A03027-1 on rat spleen for research purposes, then I may be interested in using anti-FBXL11/KDM2A antibody A03027-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Parker
Verified customer
Asked: 2017-02-27
Answer
The products we sell, including anti-FBXL11/KDM2A antibody A03027-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-02-27
Question
We are currently using anti-FBXL11/KDM2A antibody A03027-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
B. Taylor
Verified customer
Asked: 2016-05-09
Answer
The anti-FBXL11/KDM2A antibody (A03027-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-09
