This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
POU5F1 antibody (A00174) performs well in ELISA, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Immunohistochemistry of POU5F1 in rat liver tissue with POU5F1 antibody at 5 μgg/mL.
Bundle Save
Anti-POU5F1 Antibody
POU5F1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
POU5F1 antibody can be used for detection of POU5F1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-POU5F1 Antibody
View all POU5F1 Antibodies
View all POU5F1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
POU5F1 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
POU5F1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
POU5F1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human POU5F1. The immunogen is located within amino acids 260 - 310 of POU5F1.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
38.6 kDa
Background of POU5F1
POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Cite This Product
Anti-POU5F1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00174)
Publications
Application:
Species:
Yijing Decoction improves premature ovarian failure in rats by activating VEGF/VEGFR-2/FAK pathway
Published: 2025-04-26
Journal: Journal of Ovarian Research
Application: IF
Impact Factor: 4.20
Species: Rat
PMID: 40287776
Review
Have you used Anti-POU5F1 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-POU5F1 Antibody performs excellently in relevant applications for ELISA, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00174
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026