Anti-POU5F1 Antibody

Product Name

Anti-POU5F1 Antibody

View all POU5F1 Antibodies

SKU/Catalog Number

A00174

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-POU5F1 Antibody (Catalog # A00174). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

POU5F1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-POU5F1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00174)

Host

Rabbit

Contents

POU5F1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

POU5F1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human POU5F1. The immunogen is located within amino acids 260 - 310 of POU5F1.

Reactive Species

A00174 is reactive to POU5F1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

38.6 kDa

Background of POU5F1

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00174 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

POU5F1 antibody can be used for detection of POU5F1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information POU5F1

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