|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-PP2A-alpha Antibody|
|Description||Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-PP2A-alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1068)|
|Replacement Item||This antibody may replace the following items: sc-12615|sc-12615-R|sc-130237|sc-13601|sc-14020|sc-166034|sc-271903|sc-376673|sc-376824|sc-56950|sc-6110|sc-80665|sc-81601|sc-81602|sc-81603 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human PP2A-alpha(6-20aa FTKELDQWIEQLNEC), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PP2A-alpha)|
|Molecular Weight||35594 MW|
|Protein Function||PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. .|
|Subcellular Localization||Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity). .|
|Alternative Names||Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;PP2A-alpha;188.8.131.52;Replication protein C;RP-C;PPP2CA;|
|Research Areas|||cell biology|cell cycle|kinases/phosphatases| cell biology| neuroscience|neurotransmission|intracellular signaling| signal transduction|protein phosphorylation|ser / thr phosphatases| stem cells|signaling pathways|wnt|cytoplasmic| epigenetics and nuclear signaling|tgf beta| cancer| cardiovascular|heart|contractility|contractile proteins|troponin||
Background for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PP2A-alpha)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PP2A-alpha Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PP2A-alpha Antibody Images
Click the images to enlarge.
All lanes: Anti PP2A-alpha(PA1068) at 0.5ug/ml
Lane 1: Rat kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
IHC(P): Human Mammary Cancer Tissue
IHC(P): Rat Kidney Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,