Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 1 publication(s).
Anti-PP2A-alpha/PPP2CA Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-PP2A-alpha/PPP2CA Antibody
See all PP2A alpha primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for PP2A ALPHA/PPP2CA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. PP2A ALPHA/PPP2CA information: Molecular Weight: 35594 MW; Subcellular Localization: Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity).|
|Cite This Product||Anti-PP2A-alpha/PPP2CA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1068)|
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|Anti-PP2A-alpha/PPP2CA Antibody may replace the following items: sc 12615|sc 12615 R|sc 130237|sc 13601|sc 14020|sc 166034|sc 271903|sc 376673|sc 376824|sc 56950|sc 6110|sc 80665|sc 81601|sc 81602|sc 81603.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human PP2A-alpha(6-20aa FTKELDQWIEQLNEC), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For PPP2CA (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5515|
|Species Of This Entry||Human|
|Protein Name||Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform|
|Superfamily||PPP phosphatase family|
|Alternative Names||PP2A alpha|EC 220.127.116.11; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform|
|Post Tranlational Modifications||Phosphorylation; Dephosphorylation; Cleavage; Methylation; Deacetylation; Ribosylation; Ubiquitination|
|Subcellular Localization||Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGO2 (By similarity).|
|Gene Location||5q31.1, on Chromasome 5, gene sequence: NC_000005.10 (134194332..134226073, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For PPP2CA (Source: Uniprot.org, NCBI)
|Protein Function||PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.|
|Interacting Proteins||AKT1; ANP32A; EIF4E; IER3; IGBP1; Igbp1; IKBKB; IKBKG; KISS1R; Nxn; PPME1; PPP2R1A; PPP2R1B; PPP2R2C; PPP2R5A; PPP2R5B; PPP2R5C; RAF1; RBL1; RBL2; RELA; SGO1; STRIP1; STRN; TIPRL; TRIP13|
|Research Areas||Breast Cancer; Cancer; Cell Cycle and Replication; Checkpoint signaling; DNA Double Strand Break Repair; DNA Repair; Neuronal Cell Markers; Neurotransmission; Phospho-Specific; Signal Transduction|
|Related Diseases||Malignant Neoplasms; Neoplasms; Alzheimer's Disease; Cell Transformation, Neoplastic; Neurofibrillary Tangles; Leukemia; Tissue Adhesions; Leukemia, Myelocytic, Acute; Malignant Neoplasm Of Prostate; Retinoblastoma; Malignant Neoplasm Of Breast; Malignant Tumor Of Colon; Myeloid Leukemia; Lupus Erythematosus, Systemic; Mammary Neoplasms; Colonic Neoplasms; Melanoma; Hyperhomocysteinemia; Immunologic Deficiency Syndromes; Adenocarcinoma|
|Related Pathways||Localization; Cell Cycle; Cell Proliferation; Hyperphosphorylation; Cell Death; Cell Growth; Translation; Mitosis; Pathogenesis; Nuclear Export; Protein Phosphorylation; Nuclear Import; Methylation; Senescence; Dna Replication; Immune Response; Cell Cycle Arrest; Cytokinesis; Cell Differentiation; Interphase|
|Background||The catalytic subunit of human protein phosphatase 2A(PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene(approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue(Leu309) of protein serine/threonine phosphatase 2A catalytic subunit(PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.|
|Scientific References||PMID: 2849764 by Stone S.R., et al. The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A alpha catalytic subunit.|
PMID: 2837763 by Arino J., et al. Human liver phosphatase 2A: cDNA and amino acid sequence of two catalytic subunit isotypes.
Our Boster Quality Guarantee for Anti-PP2A-alpha/PPP2CA Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti PP2A-alpha(PA1068) at 0.5ug/ml
Lane 1: Rat kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
IHC(P): Human Mammary Cancer Tissue
IHC(P): Rat Kidney Tissue
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