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Product Info Summary
| SKU: | A04022-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-PPP1CB Antibody
SKU/Catalog Number
A04022-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PPP1CB Antibody catalog # A04022-2. Tested in WB,IP,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPP1CB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04022-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein corresponding to human PPP1CB aa 100-327.
Reactive Species
A04022-2 is reactive to PPP1CB in Human, Mouse, Rat
Calculated molecular weight
37.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04022-2 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:10,000
IP: 1:50
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of PPP1CB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Mouse skeletal muscle tissue lysateLane 2: Rat brain tissue lysateLane 3: A431 cell lysateLane 4: SH-SY-5Y cell lysateLane 5: SiHa cell lysateLane 6: 293 cell lysateLane 7: Hela cell lysateLane 8: PC-3M cell lysateLane 9: HepG2 cell lysateLane 10: A549 cell lysate
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Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PPP1CB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-40 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Specific Publications For Anti-PPP1CB Antibody (A04022-2)
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