Anti-Prion protein PrP/PRNP Antibody
Rabbit IgG polyclonal antibody for Major prion protein(PRNP) detection. Tested with WB, IHC-P in Mouse;Rat.
Anti-Prion protein PrP/PRNP Antibody Info At A Glance
|Product Name||Anti-Prion protein PrP/PRNP Antibody
See all Prnp primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for PRP/PRNP detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. PRP/PRNP information: Molecular Weight: 27977 MW; Subcellular Localization: Cell membrane ; Lipid-anchor, GPI-anchor . Golgi apparatus . Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU(2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis; Tissue Specificity: Highly expressed in the brain, lung, kidney and heart. Expressed at low levels in the liver and spleen.|
|Cite This Product||Anti-Prion protein PrP/PRNP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1795)|
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|Anti-Prion protein PrP/PRNP Antibody may replace the following items: sc 32304|sc 6129|sc 17833|sc 53792|sc 57072|sc 8283|sc 13950|sc 55484|sc 6128|sc 8359|sc 13950|sc 17833|sc 32303|sc 32304|sc 32305|sc 53792|sc 55484|sc 57072|sc 6128|sc 6129|sc 8283.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of mouse PRNP (143-159aa DWEDRYYRENMYRYPNQ), identical to the related rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Major prion protein(PrP)|
|Subcellular Localization||Cell membrane ; Lipid-anchor, GPI-anchor . Golgi apparatus . Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU(2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis. .|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains. .|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Our Boster Quality Guarantee for Anti-Prion protein PrP/PRNP Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti PRNP (PA1795) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 28KD
Observed bind size: 28KD
IHC(P): Rat Brain Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Note: sample size takes longer to process, average 5-7 days.
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