Product Info Summary
SKU: | PA1795 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Prion protein PrP/PRNP Antibody
View all Prion protein Antibodies
SKU/Catalog Number
PA1795
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prion protein PrP/PRNP Antibody catalog # PA1795. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prion protein PrP/PRNP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1795)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse PRNP, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1795 is reactive to PRNP in Mouse, Rat
Applications
PA1795 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
28 kDa
Calculated molecular weight
8.691kDa
Background of Prion protein
PRNP (prion protein), also known as CD230 and PRP, is a protein that in humans is encoded by the PRNP gene. The major prion protein is expressed in the brain and several other tissues. Expression is most predominant in the nervous system but occurs in many other tissues throughout the body. Puckett et al. (1991)identified a RFLP with a high degree of heterozygosity in the 5-prime region of the PRNP gene, which might serve as a useful marker for the pter-p12 region of chromosome 20. PRNP is associated with a variety of cognitive deficiencies and neurodegenerative diseases such as Creutzfeldt-Jakob disease, bovine spongiform encephalopathy, and kuru. PRNP is highly conserved through mammals, lending credence to application of conclusions from test animals such as mice. Comparison between primates is especially similar, ranging from 92.9-99.6% similarity in amino acid sequences.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-PRNP antibody, PA1795, Western blotting
All lanes: Anti PRNP (PA1795) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 28KD
Observed bind size: 28KD
Click image to see more details
Anti-PRNP antibody, PA1795, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info & Infographic
Gene/Protein Information For PRNP (Source: Uniprot.org, NCBI)
Gene Name
PRNP
Full Name
Alternative prion protein
Weight
8.691kDa
Alternative Names
CD230; CJD; fatal familial insomnia); GSS; prion protein (p27-30); prion protein PrP; prion protein; prion-related protein; PRIPMGC26679 PRNP ASCR, AltPrP, CD230, CJD, GSS, KURU, PRIP, PrP, PrP27-30, PrP33-35C, PrPc, p27-30 prion protein major prion protein|alternative prion protein|CD230 antigen|prion-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRNP, check out the PRNP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRNP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prion protein PrP/PRNP Antibody (PA1795)
Hello CJ!
PA1795 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Prion protein PrP/PRNP Antibody
Question
Is a blocking peptide available for product anti-Prion protein PrP/PRNP antibody (PA1795)?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
We do provide the blocking peptide for product anti-Prion protein PrP/PRNP antibody (PA1795). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-24
Question
I am interested in using your anti-Prion protein PrP/PRNP antibody for negative regulation of catalytic activity studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Thank you for your inquiry. This PA1795 anti-Prion protein PrP/PRNP antibody is validated on rat brain tissue, tissue lysate. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-24
Question
We are interested in to test anti-Prion protein PrP/PRNP antibody PA1795 on mouse brodmann (1909) area 23 for research purposes, then I may be interested in using anti-Prion protein PrP/PRNP antibody PA1795 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The products we sell, including anti-Prion protein PrP/PRNP antibody PA1795, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-17
Question
Do you have a BSA free version of anti-Prion protein PrP/PRNP antibody PA1795 available?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Prion protein PrP/PRNP antibody PA1795 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-02
Question
Will PA1795 anti-Prion protein PrP/PRNP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
You can see on the product datasheet, PA1795 anti-Prion protein PrP/PRNP antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-17
Question
We have tried in the past anti-Prion protein PrP/PRNP antibody for WB on prostate last year. I am using rat, and We are going to use the antibody for IHC next. I am interested in examining prostate as well as brain ovary in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
T. Carter
Verified customer
Asked: 2019-02-14
Answer
I looked at the website and datasheets of our anti-Prion protein PrP/PRNP antibody and it seems that PA1795 has been validated on rat in both WB and IHC. Thus PA1795 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-02-14
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brodmann (1909) area 23 using anti-Prion protein PrP/PRNP antibody PA1795. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-07
Question
We have observed staining in rat prostate. Any tips? Is anti-Prion protein PrP/PRNP antibody supposed to stain prostate positively?
D. Jones
Verified customer
Asked: 2018-11-20
Answer
From what I have seen in literature prostate does express PRNP. From what I have seen in Uniprot.org, PRNP is expressed in brodmann (1909) area 23, brain, blood, prostate, brain ovary, leukemic t-cell, among other tissues. Regarding which tissues have PRNP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1363802, 1678248
Brain, and Ovary, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Prostate, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2018-11-20
Question
I see that the anti-Prion protein PrP/PRNP antibody PA1795 works with IHC, what is the protocol used to produce the result images on the product page?
C. Li
Verified customer
Asked: 2018-05-18
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-18
Question
Is this PA1795 anti-Prion protein PrP/PRNP antibody reactive to the isotypes of PRNP?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
The immunogen of PA1795 anti-Prion protein PrP/PRNP antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse PRNP (143-159aa DWEDRYYRENMYRYPNQ), identical to the related rat sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-25
Question
See below the WB image, lot number and protocol we used for brodmann (1909) area 23 using anti-Prion protein PrP/PRNP antibody PA1795. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-04
Question
We are currently using anti-Prion protein PrP/PRNP antibody PA1795 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on bovine tissues as well?
J. Jones
Verified customer
Asked: 2017-05-04
Answer
The anti-Prion protein PrP/PRNP antibody (PA1795) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-04
Question
My colleagues were content with the WB result of your anti-Prion protein PrP/PRNP antibody. However we have seen positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is PRNP supposed to be expressed?
F. Williams
Verified customer
Asked: 2016-07-22
Answer
From literature, leukemic t-cell does express PRNP. Generally PRNP expresses in cell membrane. Regarding which tissues have PRNP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1363802, 1678248
Brain, and Ovary, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Prostate, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2016-07-22
Question
I was wanting to use your anti-Prion protein PrP/PRNP antibody for IHC for mouse brodmann (1909) area 23 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brodmann (1909) area 23 identification?
E. Parker
Verified customer
Asked: 2014-07-31
Answer
You can see on the product datasheet, PA1795 anti-Prion protein PrP/PRNP antibody has been validated for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brodmann (1909) area 23 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-31
Question
My question regarding product PA1795, anti-Prion protein PrP/PRNP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Walker
Verified customer
Asked: 2014-02-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1795 anti-Prion protein PrP/PRNP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-02-28
Question
Does anti-Prion protein PrP/PRNP antibody PA1795 work for IHC with brodmann (1909) area 23?
K. Zhang
Verified customer
Asked: 2013-10-14
Answer
According to the expression profile of brodmann (1909) area 23, PRNP is highly expressed in brodmann (1909) area 23. So, it is likely that anti-Prion protein PrP/PRNP antibody PA1795 will work for IHC with brodmann (1909) area 23.
Boster Scientific Support
Answered: 2013-10-14