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Pack Size:100μg/vial
Validated Species:Mouse, Rat
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Product Name Anti-ProProtein Convertase PC4 Antibody
SKU/Catalog Number PA2086
Description Rabbit IgG polyclonal antibody for Proprotein convertase subtilisin/kexin type 4(PCSK4) detection. Tested with WB in Mouse;Rat.
Cite This Product Anti-ProProtein Convertase PC4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2086)
Host Rabbit
Isotype N/A
Validated Species Mouse, Rat
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse Proprotein convertase PC4(117-133aa DPWFSKQWYMNKEIQQD), different from the related rat sequence by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Pcsk4
Protein Name Proprotein convertase subtilisin/kexin type 4
Molecular Weight 73214 MW
Protein Function Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues.
Tissue Specificity Expressed abundantly in the testis. .
Sequence Similarities Belongs to the peptidase S8 family. Furin subfamily.
Uniprot ID P29121
Alternative Names Proprotein convertase subtilisin/kexin type 4;3.4.21.-;KEX2-like endoprotease 3;Neuroendocrine convertase 3;NEC 3;Prohormone convertase 3;Pcsk4;Nec-3, Nec3;
Research Areas |cell biology|proteolysis / ubiquitin|proteolytic enzymes| cell biology|serine protease|proprotein convertases|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Proprotein convertase subtilisin/kexin type 4

PCSK4(Proprotein Convertase, Subtilisin/Kexin-Type, 4), is an enzyme that in humans is encoded by the PCSK4 gene. The gene for PC4(Pcsk4 locus) was mapped to mouse chromosome 10, close to the adipsin locus and near the gene encoding anti-mullerian hormone. Qiu et al.(2005) found that aberrant processing of pro-IGF2 by PC4 may be a cause of IUGR. PC4 cleaved pro-IGF2 to generate the intermediate processed form, IGF2(1-102). Mature IGF2(1-67), likely resulting from further processing by carboxypeptidases, is capable of activating invasive trophoblast cells through AKT phosphorylation. Inhibition of PC4 by a PC4-specific inhibitor blocked pro-IGF2 processing and reduced trophoblast cell migration.

Anti-ProProtein Convertase PC4 Antibody Images

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Anti-ProProtein Convertase PC4 Antibody
Anti-Proprotein convertase PC4 antibody, PA2086, Western blotting
All lanes: Anti Proprotein convertase PC4 (PA2086) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD
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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.