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Product Info Summary
| SKU: | PA2086 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Proprotein convertase PC4/PCSK4 Antibody Picoband®
SKU/Catalog Number
PA2086
BA3081-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Proprotein convertase PC4/PCSK4 Antibody catalog # PA2086. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Proprotein convertase PC4/PCSK4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2086)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Proprotein convertase PC4, different from the related rat sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2086 is reactive to Pcsk4 in Mouse, Rat
Observed Molecular Weight
83 kDa
Calculated molecular weight
73.2 kDa
Background of Pcsk4
PCSK4 (Proprotein Convertase, Subtilisin/Kexin-Type, 4), is an enzyme that in humans is encoded by the PCSK4 gene. The gene for PC4 (Pcsk4 locus) was mapped to mouse chromosome 10, close to the adipsin locus and near the gene encoding anti-mullerian hormone. Qiu et al. (2005) found that aberrant processing of pro-IGF2 by PC4 may be a cause of IUGR. PC4 cleaved pro-IGF2 to generate the intermediate processed form, IGF2 (1-102). Mature IGF2 (1-67), likely resulting from further processing by carboxypeptidases, is capable of activating invasive trophoblast cells through AKT phosphorylation. Inhibition of PC4 by a PC4-specific inhibitor blocked pro-IGF2 processing and reduced trophoblast cell migration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2086 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Positive Control
WB: rat brain
Validation Images & Assay Conditions
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Western blot analysis of PCSK4 using anti-PCSK4 antibody (PA2086).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK4 antigen affinity purified polyclonal antibody (Catalog # PA2086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK4 at approximately 83KD. The expected band size for PCSK4 is at 83KD.
Specific Publications For Anti-Proprotein convertase PC4/PCSK4 Antibody Picoband® (PA2086)
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4 Customer Q&As for Anti-Proprotein convertase PC4/PCSK4 Antibody Picoband®
Question
Can you help my question with product PA2086, anti-Proprotein convertase PC4/PCSK4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2086 anti-Proprotein convertase PC4/PCSK4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-04
Question
Our lab want to know about to test anti-Proprotein convertase PC4/PCSK4 antibody PA2086 on rat right testis for research purposes, then I may be interested in using anti-Proprotein convertase PC4/PCSK4 antibody PA2086 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Carter
Verified customer
Asked: 2016-12-22
Answer
The products we sell, including anti-Proprotein convertase PC4/PCSK4 antibody PA2086, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-22
Question
We are currently using anti-Proprotein convertase PC4/PCSK4 antibody PA2086 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
F. Miller
Verified customer
Asked: 2016-02-02
Answer
The anti-Proprotein convertase PC4/PCSK4 antibody (PA2086) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-02
Question
Will anti-Proprotein convertase PC4/PCSK4 antibody PA2086 work for WB with right testis?
A. Jones
Verified customer
Asked: 2013-12-11
Answer
According to the expression profile of right testis, PCSK4 is highly expressed in right testis. So, it is likely that anti-Proprotein convertase PC4/PCSK4 antibody PA2086 will work for WB with right testis.
Boster Scientific Support
Answered: 2013-12-11
