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Pcsk4 antibody (PA2086) performs well in WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of PCSK4 using anti-PCSK4 antibody (PA2086).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK4 antigen affinity purified polyclonal antibody (Catalog # PA2086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK4 at approximately 83KD. The expected band size for PCSK4 is at 83KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK4 antigen affinity purified polyclonal antibody (Catalog # PA2086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK4 at approximately 83KD. The expected band size for PCSK4 is at 83KD.
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Anti-Proprotein convertase PC4/PCSK4 Antibody Picoband®
Pcsk4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | WB |
Tested Applications
Pcsk4 antibody was positively detected by WB in:
rat brain
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Rat, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Proprotein convertase PC4/PCSK4 Antibody Picoband®
View all PCSK4 Antibodies
View all PCSK4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Proprotein convertase PC4, different from the related rat sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
83 kDa
Calculated Molecular Weight
73.2 kDa
Background of Pcsk4
PCSK4 (Proprotein Convertase, Subtilisin/Kexin-Type, 4), is an enzyme that in humans is encoded by the PCSK4 gene. The gene for PC4 (Pcsk4 locus) was mapped to mouse chromosome 10, close to the adipsin locus and near the gene encoding anti-mullerian hormone. Qiu et al. (2005) found that aberrant processing of pro-IGF2 by PC4 may be a cause of IUGR. PC4 cleaved pro-IGF2 to generate the intermediate processed form, IGF2 (1-102). Mature IGF2 (1-67), likely resulting from further processing by carboxypeptidases, is capable of activating invasive trophoblast cells through AKT phosphorylation. Inhibition of PC4 by a PC4-specific inhibitor blocked pro-IGF2 processing and reduced trophoblast cell migration.
Cite This Product
Anti-Proprotein convertase PC4/PCSK4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2086)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
94%
High Homology
Human
82%
Moderate Homology
Cow
82%
Moderate Homology
Sheep
82%
Moderate Homology
Monkey
82%
Moderate Homology
Dog
71%
Moderate Homology
Drosophila
47%
Low homology
Zebrafish
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Pcsk4 antibody
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Product Promise
We promise Anti-Proprotein convertase PC4/PCSK4 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2086
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 27, 2026