Human TLR1/TIL ELISA Kit PicoKine™

Human TLR1 / TIL ELISA Kit PicoKine™ (96 Tests). Quantitate Human TLR1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1129
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human TLR1/TIL ELISA Kit PicoKine™

See all TLR1 products

SKU/Catalog Number

EK1129

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TLR1 / TIL ELISA Kit PicoKine™ (96 Tests). Quantitate Human TLR1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TLR1/TIL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1129)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-N578

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1129 is reactive to TLR1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1129 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1129-CAP Anti-Human TLR1 Pre-coated 96-well strip microplate 1
EK1129-ST Human TLR1 Standard 2 vials, 10 ng/tube
EK1129-DA Human TLR1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0590.1880.2500.4210.7551.4001.9312.311

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TLR1, Dilution ratio of 1:1, concentration in serum is around 34pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TLR1/TIL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2041142609921810816218
Standard deviation11.2278.79378.1316.2%177.83472.56
CV (%)5.5%6.9%6.2%7.3%7.2%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TLR1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12042082031922015.932.9%
Sample 21142104510321050106743.654%
Sample 360996066552760065924231.93.9%
*number of samples for each test n=16.

Gene/Protein Information For TLR1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15399

Gene ID

7096

Gene Name

TLR1

Full Name

Toll-like receptor 1

Weight

90.291kDa

Superfamily

Toll-like receptor family

Alternative Names

CD281 antigen; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012MGC126311; MGC104956; MGC126312; rsc786; TIL; TIL. LPRS5; TLR1; Toll/interleukin-1 receptor-like protein; toll-like receptor 1 TLR1 CD281, TIL, TIL. LPRS5, rsc786 toll like receptor 1 toll-like receptor 1|toll/interleukin-1 receptor-like protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TLR1, check out the TLR1 Infographic

TLR1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TLR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1129 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Reduced B lymphocyte and immunoglobulin levels after atacicept treatment in patients with systemic lupus erythematosus: results of a multicenter, phase Ib, double %u2026

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5 Customer Q&As for Human TLR1/TIL ELISA Kit PicoKine™

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

H. Johnson

Verified customer

Asked: 2019-11-20

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-11-20

Question

Q: how to analyze ELISA data? I have obtained TLR1 level in plasma.

S. Thompson

Verified customer

Asked: 2019-01-16

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-01-16

Question

Q: is it okay to use citrate plasma as samples in Human TLR1 Picokine® ELISA Kit (Catalog # EK1129)?

Verified Customer

Verified customer

Asked: 2018-05-09

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TLR1 causing degradation of its protein structure. TLR1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TLR1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-05-09

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis TLR1 using the EK1129 Human TLR1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-02-22

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-02-22

Question

Q: can you recommend the dilution ratio of serum samples for detection of TLR1 in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

L. Varadkar

Verified customer

Asked: 2016-01-23

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TLR1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2016-01-23

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