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- Table of Contents
F2 antibody (A00044) performs well in ELISA, Flow Cytometry, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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4. Flow Cytometry analysis of H-PBMC cells using anti-Prothrombin antibody (A00044).
Overlay histogram showing H-PBMC cells stained with A00044 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Prothrombin Antibody (A00044,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing H-PBMC cells stained with A00044 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Prothrombin Antibody (A00044,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for F2? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the F2 Western Blot GuideBundle Save
Anti-Prothrombin/F2 Antibody Picoband®
F2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IHC, ICC, WB |
Tested Applications
F2 antibody was positively detected by WB in:
human plasma
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
F2 antibody was positively detected by IHC in:
human colon cancer tissue, human lung cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
F2 antibody was positively detected by FC in:
Human PBMC cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry
0.5-1μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
ELISA
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Tested
Product Information
Product Name
Anti-Prothrombin/F2 Antibody Picoband®
View all F2 Antibodies
View all F2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Prothrombin recombinant protein (Position: Y67-R124).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
85 kDa
Calculated Molecular Weight
70.0 kDa
Background of F2
F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.
Cite This Product
Anti-Prothrombin/F2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00044)
Protocols
Western Blot protocol for F2 antibody
Immunohistochemistry (IHC) protocol for F2 antibody
Flow Cytometry (FC) protocol for F2 antibody
Publications
Application:
Species:
An Apoptosis-Related Specific Risk Model for Breast Cancer: From Genomic Analysis to Precision Medicine
Published: 2024-06-27
Journal: Frontiers in Bioscience-Landmark
Application: IHC
Impact Factor: 3.30
Species: Human
PMID: 39082332
Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma
Published: 2021-02-25
Journal: Frontiers in Oncology
Application: IHC
Impact Factor: 6.24
Species: Human
PMID: 33718182
Review
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Product Promise
We promise Anti-Prothrombin/F2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00044
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026