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Product Info Summary
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Boster Bio Anti-Prothrombin/F2 Antibody catalog # PA2059. Tested in WB applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prothrombin/F2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2059)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of rat Prothrombin (600-612aa THVFRLKRWMQKV).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA2059 is reactive to F2 in Rat
PA2059 is guaranteed for WB Boster Guarantee
Background of Coagulation Factor II/Thrombin
F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. Royle et al. (1987) assigned the gene for human prothrombin (F2) to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Anti-F2 antibody, PA2059, Western blotting
All lanes: Anti F2 (PA2059) at 0.5ug/ml
WB : Rat Testis Tissue Lysate at 50ug
Predicted bind size: 70KD
Observed bind size: 100KD
Gene/Protein Information For F2 (Source: Uniprot.Org, NCBI)
peptidase S1 family
coagulation factor II (thrombin) receptor-like 2; Coagulation factor II receptor-like 2 (protease-actovated receptor 3); Coagulation factor II receptor-like 2; Coagulation Factor II; F2; PAR-3; PAR3proteinase-activated receptor 3; protease-activated receptor 3; proteinase-activated receptor-3; PT; serine protease; Thrombin receptor-like 2; Thrombin F2|coagulation factor II|prothrombin|coagulation factor 2|thrombin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on F2, check out the F2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA2059 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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