Anti-Prothrombin/F2 Antibody

Coagulation Factor II/Thrombin antibody

Boster Bio Anti-Prothrombin/F2 Antibody catalog # PA2059. Tested in WB applications. This antibody reacts with Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA2059
Size: 100μg/vial
Reactive Species: Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Prothrombin/F2 Antibody

View all Coagulation Factor II/Thrombin Antibodies

SKU/Catalog Number

PA2059

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Prothrombin/F2 Antibody catalog # PA2059. Tested in WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Prothrombin/F2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2059)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat Prothrombin (600-612aa THVFRLKRWMQKV).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA2059 is reactive to F2 in Rat

Applications

PA2059 is guaranteed for WB Boster Guarantee

Background of Coagulation Factor II/Thrombin

F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. Royle et al. (1987) assigned the gene for human prothrombin (F2) to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For F2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P18292

Gene ID

29251

Gene Name

F2

Full Name

Prothrombin

Weight

70.412kDa

Superfamily

peptidase S1 family

Alternative Names

coagulation factor II (thrombin) receptor-like 2; Coagulation factor II receptor-like 2 (protease-actovated receptor 3); Coagulation factor II receptor-like 2; Coagulation Factor II; F2; PAR-3; PAR3proteinase-activated receptor 3; protease-activated receptor 3; proteinase-activated receptor-3; PT; serine protease; Thrombin receptor-like 2; Thrombin F2|coagulation factor II|prothrombin|coagulation factor 2|thrombin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on F2, check out the F2 Infographic

F2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for F2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA2059 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Heparin Impairs Angiogenesis through Inhibition of MicroRNA-10b

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16 Customer Q&As for Anti-Prothrombin/F2 Antibody

Question

My question regarding product PA2059, anti-Prothrombin/F2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Miller

Verified customer

Asked: 2020-04-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2059 anti-Prothrombin/F2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-15

Question

My team were content with the WB result of your anti-Prothrombin/F2 antibody. However we have observed positive staining in liver extracellular space. using this antibody. Is that expected? Could you tell me where is F2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

According to literature, liver does express F2. Generally F2 expresses in secreted, extracellular space. Regarding which tissues have F2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 14962227
Cerebrospinal fluid, Pubmed ID: 19838169
Liver, Pubmed ID: 15489334, 19159218, 24275569
Liver, and Mammary gland, Pubmed ID: 14702039
Plasma, Pubmed ID: 14760718, 16335952
Urine, Pubmed ID: 8073540

Boster Scientific Support

Answered: 2020-02-14

Question

We need using your anti-Prothrombin/F2 antibody for r-rno-140837; intrinsic pathway of fibrin clot formation studies. Has this antibody been tested with western blotting on rat testis tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

Thank you for your inquiry. This PA2059 anti-Prothrombin/F2 antibody is validated on rat testis tissue, tissue lysate. It is guaranteed to work for WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-09-04

Question

Our lab want to know about to test anti-Prothrombin/F2 antibody PA2059 on rat liver for research purposes, then I may be interested in using anti-Prothrombin/F2 antibody PA2059 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

The products we sell, including anti-Prothrombin/F2 antibody PA2059, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-05

Question

Will anti-Prothrombin/F2 antibody PA2059 work for WB with liver?

J. Roberts

Verified customer

Asked: 2019-05-07

Answer

According to the expression profile of liver, F2 is highly expressed in liver. So, it is likely that anti-Prothrombin/F2 antibody PA2059 will work for WB with liver.

Boster Scientific Support

Answered: 2019-05-07

Question

We are currently using anti-Prothrombin/F2 antibody PA2059 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-02-12

Answer

The anti-Prothrombin/F2 antibody (PA2059) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-12

Question

I see that the anti-Prothrombin/F2 antibody PA2059 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-02-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-02-05

Question

Is a blocking peptide available for product anti-Prothrombin/F2 antibody (PA2059)?

Verified Customer

Verified customer

Asked: 2017-11-14

Answer

We do provide the blocking peptide for product anti-Prothrombin/F2 antibody (PA2059). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-14

Question

Would anti-Prothrombin/F2 antibody PA2059 work on feline WB with right lobe of liver?

L. Miller

Verified customer

Asked: 2017-05-30

Answer

Our lab technicians have not tested anti-Prothrombin/F2 antibody PA2059 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Prothrombin/F2 antibody PA2059 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline right lobe of liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-05-30

Question

Please see the WB image, lot number and protocol we used for liver using anti-Prothrombin/F2 antibody PA2059. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-24

Question

We have seen staining in rat blood. What should we do? Is anti-Prothrombin/F2 antibody supposed to stain blood positively?

O. Gonzalez

Verified customer

Asked: 2017-02-28

Answer

Based on literature blood does express F2. Based on Uniprot.org, F2 is expressed in right lobe of liver, blood, liver mammary gland, liver, urine, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have F2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 14962227
Cerebrospinal fluid, Pubmed ID: 19838169
Liver, Pubmed ID: 15489334, 19159218, 24275569
Liver, and Mammary gland, Pubmed ID: 14702039
Plasma, Pubmed ID: 14760718, 16335952
Urine, Pubmed ID: 8073540

Boster Scientific Support

Answered: 2017-02-28

Question

Is this PA2059 anti-Prothrombin/F2 antibody reactive to the isotypes of F2?

A. Parker

Verified customer

Asked: 2016-12-06

Answer

The immunogen of PA2059 anti-Prothrombin/F2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of rat Prothrombin(600-612aa THVFRLKRWMQKV). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-12-06

Question

Would PA2059 anti-Prothrombin/F2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Parker

Verified customer

Asked: 2016-10-11

Answer

It shows on the product datasheet, PA2059 anti-Prothrombin/F2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-10-11

Question

Is there a BSA free version of anti-Prothrombin/F2 antibody PA2059 available?

E. Lewis

Verified customer

Asked: 2014-06-03

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Prothrombin/F2 antibody PA2059 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-06-03

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Prothrombin/F2 antibody PA2059. Let me know if you need anything else.

B. Roberts

Verified customer

Asked: 2013-11-15

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-11-15

Question

I was wanting to use your anti-Prothrombin/F2 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?

K. Zhao

Verified customer

Asked: 2013-09-24

Answer

You can see on the product datasheet, PA2059 anti-Prothrombin/F2 antibody has been validated for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-09-24

Size

Total: $315

SKU:PA2059

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