BBC3 Antibodies

AND BBC3 ELISA kits, BBC3 Proteins

Many biological assays use antibodies to detect BBC3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with BBC3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop BBC3 antibodies...

We validate the specificity of these antibodies to BBC3 by testing them on tissues known to express BBC3 positively and negatively. Browse below to find the BBC3 antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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BBC3 Infographic by Boster Bio

All BBC3 Products

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Figures

Specificity

Applications

Price

Anti-PUMA/BBC3 Antibody Picoband®

3 Q&As

Anti-PUMA antibody, PA1313, Western blottingAll lanes: Anti PUMA(PA1313) at 0

Human, Mouse, Rat

$370
Cat # PA1313
100 μg/vial

Anti-PUMA BBC3 Rabbit Monoclonal Antibody

3 Citations

Western blot analysis of PUMA using anti-PUMA antibody (M04899)

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PUMA Antibody

Immunofluorescent analysis of Hela cells, using PUMA Antibody

Human, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M04899
100 μl

Anti-PUMA BBC3 Antibody

Western Blot Validation of PUMA in K562 and 3T3/NIH Cells
Loading: 15 μg of lysates per lane

Validation with PUMA siRNA Knockdown in 293 Cells
293 cells were transfected with control siRNAs (lane 1) or PUMA siRNAs (lane 2)
Loading: 15 μg of 293 whole cell lysates per lane

Human, Mouse

ELISA, IF, ICC, WB

$481
Cat # A04899
0.1 mg

Anti-PUMA BBC3 Antibody

Western Blot Validation of PUMA in K562 Cells
Loading: 15 μg of lysates per lane

Immunofluorescence Validation of PUMA in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with A04899-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue)

Human, Rat

ELISA, IF, IHC-P, WB

$481
Cat # A04899-1
0.1 mg

Anti-PUMA/BBC3 Antibody Picoband®

Western blot analysis of PUMA/BBC3 using anti-PUMA/BBC3 antibody (A04899-3)

Human

$370
Cat # A04899-3
100 μg/vial

Anti-PUMA Rabbit Monoclonal Antibody

Human hepatocellular carcinoma was stained with anti-PUMA rabbit antibody

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PUMA antibody

Human, Mouse, Rat

ELISA, IP, IF, IHC, WB

$146
Cat # M04899-2
20μL, 40μL, 100μL, 200μL

BBC3 Overview

Facts about Bcl-2-binding component 3, isoforms 3/4.

BBC3 3D structure. Source: alphafold

Bcl-2-binding component 3, isoforms 3/4 (BBC3)

Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to reveal some growth- inhibitory or apoptotic activity.

Gene Information

Human
Gene Name:	BBC3
Uniprot:	Q96PG8
Entrez:	27113

Family

Belongs to:

No superfamily

Alternative Names

BCL2 binding component 3; bcl-2-binding component 3; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMAFLJ42994

Molecular Weight

Mass (kDA):
26.498 kDA

Genomic Context


Human

Location:	19q13.32
Sequence:	19; NC_000019.10 (47220822..47232998, complement)

Subcellular Localizations

Contrary to isoforms 1 and 2, isoform 3 does not localize to the mitochondria.

Diseases

Malignant Neoplasms
Neoplasms
Carcinoma
Leukemia
Malignant Tumor Of Colon
Cell Transformation, Neoplastic
Malignant Neoplasm Of Breast
Colorectal Cancer
Mammary Neoplasms
Colorectal Neoplasms
Malignant Paraganglionic Neoplasm
Colonic Neoplasms

Neuroblastoma
Melanoma
Adenocarcinoma
Lymphoma
Lymphoid Leukemia
Lung Neoplasms
Genotoxic Stress

Pathways

Cell Death
Cell Cycle
Induction Of Apoptosis
Cell Cycle Arrest
Cell Growth
Rna Interference
Cell Proliferation
Mitochondrial Translocation
Pathogenesis
Sensitization
Methylation
Localization
Autophagy

Oncogenesis
Programmed Cell Death
Cell Differentiation
Dna Repair
Senescence
Apoptotic Process
Mitochondrial Outer Membrane Permeabilization

PTMs

Phosphorylation
Cleavage
Acetylation
Methylation
Ubiquitination

Sumoylation
Deacetylation
Farnesylation
Glycosylation
Dephosphorylation

Research Areas

Apoptosis
Cancer
DNA Double Strand Break Repair
Signal Transduction
Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BBC3

References

PMID: 11463392 by Nakano K., et al. PUMA, a novel proapoptotic gene, is induced by p53.

2 Publications of Boster BBC3 Antibodies & ELISA Kits

PubMed ID: 25630785, Nerve growth factor reduces apoptotic cell death in rat facial motor neurons aft...
PubMed ID: 21248471, Zhao Y, Wu S, Wu J, Jia P, Gao S, Yan X, Wang Y. Cancer Biol Ther. 2011 Jan 1;11...

*More publications can be found for each product on its corresponding product page