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Product Info Summary
| SKU: | A02911 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Rab 6A Antibody
SKU/Catalog Number
A02911
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rab 6A Antibody catalog # A02911. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab 6A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02911)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RAB6A. AA range:113-162
Reactive Species
A02911 is reactive to RAB6A in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
23.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02911 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of COLO cells using Rab 6A Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from A549 cells, using RAB6A Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HT-29 cells using RAB6A antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Specific Publications For Anti-Rab 6A Antibody (A02911)
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Customer Q&As
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3 Customer Q&As for Anti-Rab 6A Antibody
Question
Is this A02911 anti-Rab 6A antibody reactive to the isotypes of RAB6A?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The immunogen of A02911 anti-Rab 6A antibody is Synthesized peptide derived from the Internal region of human Rab 6A. at AA rangle: 90-170. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-08
Question
Can you help my question with product A02911, anti-Rab 6A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02911 anti-Rab 6A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-29
Question
Is a blocking peptide available for product anti-Rab 6A antibody (A02911)?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
We do provide the blocking peptide for product anti-Rab 6A antibody (A02911). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-22
