Human Decorin / DCN ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human Decorin / DCN ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human DCN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ0749
Size: For 5 plates, 96 wells each, reagents only, plates are not included
Reactive Species: Human
Application: ELISA

Product Name

Human Decorin / DCN ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all DCN products

SKU/Catalog Number

EZ0749

Size

For 5 plates, 96 wells each, reagents only, plates are not included

*Question: How many samples can I assay/run in this kit?

Description

Human Decorin / DCN ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human DCN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Decorin / DCN ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0749)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D31-K359

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0749 is reactive to DCN in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ0749 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0749-CA Mouse anti- human DCN monoclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0749-DA Biotinylated mouse anti- human DCN monoclonal antibody (Detection Antibody) 500 µl, 10.1 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0749-ST Lyophilized recombinant human DCN standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0010.0630.1290.3220.6201.1241.7972.259

Data Example Images

Gene/Protein Information For DCN (Source: Uniprot.Org, NCBI)

Uniprot ID

P07585

Gene ID

1634

Gene Name

DCN

Full Name

Decorin

Weight

39.747kDa

Superfamily

small leucine-rich proteoglycan (SLRP) family

Alternative Names

Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B DCN CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B decorin decorin|bone proteoglycan II|dermatan sulphate proteoglycans II|proteoglycan core protein|small leucine-rich protein 1B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DCN, check out the DCN Infographic

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In this infographic you will see the following information for DCN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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1 Customer Q&As for Human Decorin / DCN ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

Was baculovirus used in the EZ0749 standard to introduce the recombinant gene into cells?

Verified customer

Asked: 2020-11-20

Answer

For the standard in the Human Decorin / DCN ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0749), baculovirus was not used to introduce the recombinant gene. The gene is Decorin/DCN only.

Boster Scientific Support

Answered: 2020-11-20

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