Click image to see more details
Product Info Summary
| SKU: | M00204 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Ran Monoclonal Antibody
SKU/Catalog Number
M00204
BM5298 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ran Monoclonal Antibody catalog # M00204. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ran Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00204)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACFI-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ran GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity) .
Reactive Species
M00204 is reactive to RAN in Human, Mouse, Rat
Observed Molecular Weight
24 kDa
Calculated molecular weight
24.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00204 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
FC 1:20
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Ran expression in (1)HeLa cell lysate; (2)RAW264.7 cell lysate.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:500 dilution.
Specific Publications For Anti-Ran Monoclonal Antibody (M00204)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Ran Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Ran Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-Ran Monoclonal Antibody
Question
We are currently using anti-Ran Monoclonal antibody M00204 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
S. Jackson
Verified customer
Asked: 2020-04-30
Answer
The anti-Ran Monoclonal antibody (M00204) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
Question
We have been able to see staining in human erythroleukemia. What should we do? Is anti-Ran Monoclonal antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
According to literature erythroleukemia does express RAN. According to Uniprot.org, RAN is expressed in testis, teratocarcinoma, brain, umbilical cord blood, hippocampus, lymph, ovary, skin uterus, platelet, brain cajal-retzius cell, colon adenocarcinoma, cervix carcinoma, lymphoblast, melanoma, erythroleukemia, liver, among other tissues. Regarding which tissues have RAN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8421051, 10400640
Cervix carcinoma, Pubmed ID: 1961752
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymph, Ovary, Skin, and Uterus, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Teratocarcinoma, Pubmed ID: 2108320
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2018-07-11
Question
We ordered your anti-Ran Monoclonal antibody for IF on brain cajal-retzius cell in the past. I am using rat, and We are going to use the antibody for WB next. My question regards examining brain cajal-retzius cell as well as skin uterus in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
I looked at the website and datasheets of our anti-Ran Monoclonal antibody and it appears that M00204 has been validated on rat in both IF and WB. Thus M00204 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-05
Question
My team were happy with the WB result of your anti-Ran Monoclonal antibody. However we have been able to see positive staining in hippocampus nucleus using this antibody. Is that expected? Could you tell me where is RAN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
According to literature, hippocampus does express RAN. Generally RAN expresses in nucleus. Regarding which tissues have RAN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8421051, 10400640
Cervix carcinoma, Pubmed ID: 1961752
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymph, Ovary, Skin, and Uterus, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Teratocarcinoma, Pubmed ID: 2108320
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2017-11-20
