Product Info Summary
| SKU: | PA2163 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RGS14 Antibody Picoband®
SKU/Catalog Number
PA2163
BA3100-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RGS14 Antibody catalog # PA2163. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RGS14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2163)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RGS14, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2163 is reactive to RGS14 in Human, Mouse, Rat
Observed Molecular Weight
15 kDa
Calculated molecular weight
61.4 kDa
Background of RGS14
Regulator of G-protein signaling 14, also called RGS14, is a protein that in humans is encoded by the RGS14 gene. This gene encodes a member of the regulator of G-protein signaling family. By genomic sequence analysis, this gene is mapped to chromosome 5q35.3. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2163 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Thymus Tissue, Rat Spleen Tissue, RAJI Cell, CEM Cell, JURKAT Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-RGS14 antibody, PA2163, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: RAJI Cell Lysate
Lane 4: CEM Cell Lysate
Lane 5: JURKAT Cell Lysate
Specific Publications For Anti-RGS14 Antibody Picoband® (PA2163)
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Customer Q&As
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5 Customer Q&As for Anti-RGS14 Antibody Picoband®
Question
Does anti-RGS14 antibody PA2163 work for WB with caudate nucleus?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
According to the expression profile of caudate nucleus, RGS14 is highly expressed in caudate nucleus. So, it is likely that anti-RGS14 antibody PA2163 will work for WB with caudate nucleus.
Boster Scientific Support
Answered: 2019-11-18
Question
We want to test anti-RGS14 antibody PA2163 on human caudate nucleus for research purposes, then I may be interested in using anti-RGS14 antibody PA2163 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The products we sell, including anti-RGS14 antibody PA2163, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-24
Question
Here is the WB image, lot number and protocol we used for caudate nucleus using anti-RGS14 antibody PA2163. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-15
Question
We are currently using anti-RGS14 antibody PA2163 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
The anti-RGS14 antibody (PA2163) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-25
Question
I see that the anti-RGS14 antibody PA2163 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-29


