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Product Info Summary
| SKU: | A00207-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Rho A Antibody
SKU/Catalog Number
A00207-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rho A Antibody catalog # A00207-1. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rho A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00207-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide of human RhoA(NP_001655.1).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00207-1 is reactive to RHOA in Human, Mouse, Rat
Calculated molecular weight
21.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00207-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using RhoA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.
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Immunohistochemistry of paraffin-embedded human placenta using RhoA Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells using 3ug RhoA antibody . Western blot was performed from the immunoprecipitate using RhoA antibody at a dilution of 1:500.
Specific Publications For Anti-Rho A Antibody (A00207-1)
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