Product Info Summary
SKU: | A04055-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-ACCN1/ASIC2 Antibody Picoband™
SKU/Catalog Number
A04055-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ACCN1/ASIC2 Antibody Picoband™ catalog # A04055-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACCN1/ASIC2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04055-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ACCN1/ASIC2 recombinant protein (Position: H27-C512).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04055-1 is reactive to ASIC2 in Human, Mouse, Rat
Applications
A04055-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
58 kDa
Calculated molecular weight
57.709kDa
Background of ACCN1
Amiloride-sensitive cation channel 1, neuronal, also known as ASIC2, is a protein that in humans is encoded by the ACCN1 gene. This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ASIC2 using anti-ASIC2 antibody (A04055-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: rat ovarian tissue lysates,
Lane 8: mouse brain tissue lysates,
Lane 9: mouse liver tissue lysates,
Lane 10: mouse ovarian tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC2 antigen affinity purified polyclonal antibody (Catalog # A04055-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC2 at approximately 58KD. The expected band size for ASIC2 is at 58KD.
Click image to see more details
Figure 2. IF analysis of ASIC2 using anti-ASIC2 antibody (A04055-1).
ASIC2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ASIC2 Antibody (A04055-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of A549 cells using anti-ASIC2 antibody (A04055-1).
Overlay histogram showing A549 cells stained with A04055-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC2 Antibody (A04055-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 4. Flow Cytometry analysis of PC-3 cells using anti-ASIC2 antibody (A04055-1).
Overlay histogram showing PC-3 cells stained with A04055-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC2 Antibody (A04055-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ASIC2 (Source: Uniprot.org, NCBI)
Gene Name
ASIC2
Full Name
Acid-sensing ion channel 2
Weight
57.709kDa
Superfamily
amiloride-sensitive sodium channel (TC 1.A.6) family
Alternative Names
Acid-sensing ion channel 2; Amiloride-sensitive brain sodium channel; amiloride-sensitive cation channel 1, neuronal; ASIC2a; ASIC2Mammalian degenerin homolog; BNAC1; BNaC1ACCN; BNC1Amiloride-sensitive cation channel neuronal 1; degenerin; hBNaC1; MDEGBrain sodium channel 1; neuronal amiloride-sensitive cation channel 1 ASIC2 ACCN, ACCN1a, BNC1, BNaC1, MDEG, hBNaC1, ASIC2 acid sensing ion channel subunit 2 acid-sensing ion channel 2|acid sensing (proton gated) ion channel 2|amiloride-sensitive cation channel 1, neuronal|brain sodium channel 1|mammalian degenerin homolog|neuronal amiloride-sensitive cation channel 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ASIC2, check out the ASIC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ASIC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACCN1/ASIC2 Antibody Picoband™ (A04055-1)
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