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- Table of Contents
ASIC2 antibody (A04055-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of A549 cells using anti-ASIC2 antibody (A04055-1).
Overlay histogram showing A549 cells stained with A04055-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC2 Antibody (A04055-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A549 cells stained with A04055-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC2 Antibody (A04055-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot or IHC for ASIC2? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-ACCN1/ASIC2 Antibody Picoband®
ASIC2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ASIC2 antibody was positively detected by WB in:
human placenta tissue, human PC-3 whole cell, human A549 whole cell, human U2OS whole cell, rat brain tissue, rat liver tissue, rat ovarian tissue, mouse brain tissue, mouse liver tissue, mouse ovarian tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ASIC2 antibody was positively detected by IHC in:
human brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ASIC2 antibody was positively detected by ICC/IF in:
A431 cell
ASIC2 antibody was positively detected by FC in:
A549 cell, PC-3 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-ACCN1/ASIC2 Antibody Picoband®
View all ASIC2 Antibodies
View all ASIC2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ACCN1/ASIC2 recombinant protein (Position: H27-C512).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated Molecular Weight
57.7 kDa
Background of ASIC2
Amiloride-sensitive cation channel 1, neuronal, also known as ASIC2, is a protein that in humans is encoded by the ACCN1 gene. This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified.
Cite This Product
Anti-ACCN1/ASIC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04055-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
99%
High Homology
Rat
99%
High Homology
Rabbit
99%
High Homology
Dog
99%
High Homology
Monkey
81%
Moderate Homology
Cow
81%
Moderate Homology
Sheep
81%
Moderate Homology
Zebrafish
76%
Moderate Homology
Drosophila
23%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ASIC2 antibody
Immunohistochemistry (IHC) protocol for ASIC2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ASIC2 antibody
Flow Cytometry (FC) protocol for ASIC2 antibody
Publications
Application:
Species:
Amiloride alleviates morphine tolerance by suppressing ASIC3-dependent neuroinflammation in the spinal cord
Published: 2023-10-31
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 5.00
Species: Mouse
PMID: 37918499
Review
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Product Promise
We promise Anti-ACCN1/ASIC2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A04055-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 30, 2026