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Product Info Summary
| SKU: | A04223-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Peptide YY/PYY Antibody
SKU/Catalog Number
A04223-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Peptide YY/PYY Antibody catalog # A04223-1. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Peptide YY/PYY Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04223-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Peptide YY/PYY, which shares 91.7% amino acid (aa) sequence identity with both mouse and rat Peptide YY/PYY.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04223-1 is reactive to PYY in Human
Calculated molecular weight
11.1 kDa
Background of PYY
Peptide YY (PYY), also known as peptide tyrosine tyrosine, is a peptide that in humans is encoded by the PYY gene. This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded preproprotein is proteolytically processed to generate two alternative peptide products that differ in length by three amino acids. These peptides, secreted by endocrine cells in the gut, exhibit different binding affinities for each of the neuropeptide Y receptors. Binding of the encoded peptides to these receptors mediates regulation of pancreatic secretion, gut mobility and energy homeostasis. Rare variations in this gene could increase susceptibility to obesity and elevated serum levels of the encoded peptides may be associated with anorexia nervosa.
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Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04223-1 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Positive Control
IHC: human appendicitis tissue
Validation Images & Assay Conditions
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IHC analysis of Peptide YY/PYY using anti-Peptide YY/PYY antibody (A04223-1). Peptide YY/PYY was detected in paraffin-embedded section of human appendicitis tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peptide YY/PYY Antibody (A04223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of Peptide YY/PYY using anti-Peptide YY/PYY antibody (A04223-1).
Peptide YY/PYY was detected in a paraffin-embedded section of human small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Peptide YY/PYY Antibody (A04223-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Peptide YY/PYY Antibody (A04223-1)
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12 Customer Q&As for Anti-Peptide YY/PYY Antibody
Question
My question regarding product A04223-1, anti-Peptide YY/PYY antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04223-1 anti-Peptide YY/PYY antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-21
Question
Would A04223-1 anti-Peptide YY/PYY antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Williams
Verified customer
Asked: 2019-11-22
Answer
You can see on the product datasheet, A04223-1 anti-Peptide YY/PYY antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-22
Question
Is a blocking peptide available for product anti-Peptide YY/PYY antibody (A04223-1)?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We do provide the blocking peptide for product anti-Peptide YY/PYY antibody (A04223-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-24
Question
Is this A04223-1 anti-Peptide YY/PYY antibody reactive to the isotypes of PYY?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
The immunogen of A04223-1 anti-Peptide YY/PYY antibody is A synthetic peptide corresponding to a sequence of human Peptide YY/PYY (YPIKPEAPREDASPEELNRYYASLRHYLNLVTRQRY). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-13
Question
Do you have a BSA free version of anti-Peptide YY/PYY antibody A04223-1 available?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Peptide YY/PYY antibody A04223-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-23
Question
I was wanting to use your anti-Peptide YY/PYY antibody for IHC for human colon mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon mucosa identification?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
As indicated on the product datasheet, A04223-1 anti-Peptide YY/PYY antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-14
Question
I have attached the WB image, lot number and protocol we used for colon mucosa using anti-Peptide YY/PYY antibody A04223-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon mucosa using anti-Peptide YY/PYY antibody A04223-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-12
Question
Does anti-Peptide YY/PYY antibody A04223-1 work for IHC with colon mucosa?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
According to the expression profile of colon mucosa, PYY is highly expressed in colon mucosa. So, it is likely that anti-Peptide YY/PYY antibody A04223-1 will work for IHC with colon mucosa.
Boster Scientific Support
Answered: 2018-04-09
Question
I see that the anti-Peptide YY/PYY antibody A04223-1 works with IHC, what is the protocol used to produce the result images on the product page?
R. Jones
Verified customer
Asked: 2016-11-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-11-02
Question
We are interested in to test anti-Peptide YY/PYY antibody A04223-1 on human colon mucosa for research purposes, then I may be interested in using anti-Peptide YY/PYY antibody A04223-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Mitchell
Verified customer
Asked: 2015-10-08
Answer
The products we sell, including anti-Peptide YY/PYY antibody A04223-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-10-08
Question
Will anti-Peptide YY/PYY antibody A04223-1 work on bovine IHC with mucosa of transverse colon?
D. Jackson
Verified customer
Asked: 2014-12-25
Answer
Our lab technicians have not tested anti-Peptide YY/PYY antibody A04223-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Peptide YY/PYY antibody A04223-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine mucosa of transverse colon in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-25
