Anti-Rim2 RIMS2 Antibody

Product Name

Anti-Rim2 RIMS2 Antibody

View all RIMS2 Antibodies

SKU/Catalog Number

A09915-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Rim2 RIMS2 Antibody (Catalog # A09915-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Rim2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Rim2 RIMS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09915-1)

Host

Rabbit

Contents

Rim2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rim2 antibody was raised against a 17 amino acid synthetic peptide near the center of the human Rim2. The immunogen is located within amino acids 250 - 300 of Rim2.

Cross-reactivity

Multiple isoforms of RIM2 are known to exist. This antibody is predicted to have no cross-reactivity to other Rim proteins.

Reactive Species

A09915-1 is reactive to RIMS2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

160.4 kDa

Background of RIMS2

Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. Rim1 and Rim2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like Rim1, Rim 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09915-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Rim2 antibody can be used for detection of Rim2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RIMS2

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