Anti-Rim3 RIMS3 Antibody

Product Name

Anti-Rim3 RIMS3 Antibody

View all RIMS3 Antibodies

SKU/Catalog Number

A13058

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Rim3 RIMS3 Antibody (Catalog # A13058). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Rim3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Rim3 RIMS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13058)

Host

Rabbit

Contents

Rim3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rim3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Rim3. The immunogen is located within amino acids 60 - 110 of Rim3.

Cross-reactivity

This antibody is predicted to have no cross-reactivity to other Rim proteins.

Reactive Species

A13058 is reactive to RIMS3 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

32.8 kDa

Background of RIMS3

Rab3-interacting molecules (RIMs) are synaptic proteins are synaptic proteins necessary for neural transmission and plasticity. While both Rim1 and Rim 2 are thought to be effector proteins for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner, less is known of Rim3. Expression of Rim3 in PC12 cells induced a significant increase in calcium-triggered exocytosis, with no appreciable change in the baseline release, suggesting that it plays a role in the regulation of exocytosis. Rim3 protein localizes primarily to neuronal dendrites and the postsynaptic densities, as opposed to Rim1 which is found in presynapse locations, indicating that Rim3 may contribute to synapse transmission and plasticity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A13058 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Rim3 antibody can be used for detection of Rim3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RIMS3

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