Anti-RLTPR Purified CARMIL2 Monoclonal Antibody

Product Name

Anti-RLTPR Purified CARMIL2 Monoclonal Antibody

View all CARMIL2 Antibodies

SKU/Catalog Number

M10789

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RLTPR Purified CARMIL2 Monoclonal Antibody (Catalog# M10789). Tested in Flow Cytometry, WB application(s). This antibody reacts with Mouse, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-RLTPR Purified CARMIL2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10789)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

EM-53

Isotype

Mouse IgG1 kappa

Immunogen

Murine RLTPR. The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M10789 is reactive to CARMIL2 in Human, Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

154.7 kDa

Background of CARMIL2

RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M10789 is guaranteed for Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 µg/ml. Intracellular staining.

Validation Images & Assay Conditions

See full target information CARMIL2

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