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Product Info Summary
| SKU: | M08956 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RNF14 Mouse Monoclonal Antibody [Clone ID: OTI1C9]
SKU/Catalog Number
M08956
Size
100 µl
Description
Boster Bio RNF14 mouse monoclonal antibody,clone OTI1C9. Catalog# M08956. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-RNF14 Mouse Monoclonal Antibody [Clone ID: OTI1C9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08956)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1C9
Isotype
IgG2a
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-220 of human RNF14 (NP_899647) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08956 is reactive to RNF14 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
54 kDa
Calculated molecular weight
53.837kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08956 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF14 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF14 (1:2000).
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Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-RNF14 monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RNF14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNF14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Protein Target Info & Infographic
Gene/Protein Information For RNF14 (Source: Uniprot.org, NCBI)
Gene Name
RNF14
Full Name
E3 ubiquitin-protein ligase RNF14
Weight
53.837kDa
Superfamily
RBR family
Alternative Names
Androgen receptor-associated protein 54; ARA54; ARA54androgen receptor associated protein 54; EC 6.3.2.-; HFB30; HFB30E3 ubiquitin-protein ligase RNF14; ring finger protein 14FLJ26004; RNF14; Triad2 protein; TRIAD2 RNF14 ARA54, HFB30, HRIHFB2038, TRIAD2 ring finger protein 14 E3 ubiquitin-protein ligase RNF14|androgen receptor associated protein 54
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF14, check out the RNF14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF14 Mouse Monoclonal Antibody [Clone ID: OTI1C9] (M08956)
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