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Product Info Summary
| SKU: | A05130 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, WB |
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Product info
Product Name
Anti-FXR2 (E576) Antibody
SKU/Catalog Number
A05130
Size
100ul
Form
Liquid
Description
Boster Bio Anti-FXR2 (E576) Antibody catalog # A05130. Tested in WB,IHC,IF,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FXR2 (E576) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05130)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 550-600 of Human FXR2.
Reactive Species
A05130 is reactive to FXR2 in Human, Mouse, Rat
Observed Molecular Weight
111 kDa
Calculated molecular weight
74.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05130 is guaranteed for IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC/IF: 1:50-1:200
IP: 1:10-1:100
Validation Images & Assay Conditions
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Western blot (WB) analysis of FXR2 (E576) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug) Lane4:MEF whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of FXR2 (E576) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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Immunoprecipitation of HEK293T cell lysate using FXR2 (E576) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using FXR2 (E576) #BS2008.“↑”（supernatant）; “↓(deposition）
Specific Publications For Anti-FXR2 (E576) Antibody (A05130)
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