RNF14 Antibodies

AND RNF14 ELISA kits, RNF14 Proteins

Many biological assays use antibodies to detect RNF14, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RNF14 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RNF14 antibodies...

We validate the specificity of these antibodies to RNF14 by testing them on tissues known to express RNF14 positively and negatively. Browse below to find the RNF14 antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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RNF14 Infographic by Boster Bio

All RNF14 Products

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Specificity

Applications

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Anti-RNF14/Ara54 Antibody

1 Q&As

Western blot analysis of lysates from RAW264

Human, Mouse, Rat

ELISA, WB

$335
Cat # A08956
100ul

RNF14 (NM_004290) Human Recombinant Protein

Coomassie blue staining of purified RNF14 protein

$1249
Cat # PROTQ9UBS8
20 µg

Anti-RNF14 Mouse Monoclonal Antibody [Clone ID: OTI1C9]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF14 (Right lane) cDNA for 48 hrs and lysed

Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-RNF14 monoclonal antibody (1:500)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RNF14 mouse monoclonal antibody

Human, Mouse, Rat

IHC, WB

$523
Cat # M08956
100 µl

Anti-RNF14 Rabbit Monoclonal Antibody

Western blot analysis of RNF14 expression in Jurkat cell lysate

Human, Mouse, Rat

$370
Cat # M08956-1
100 μl/vial

RNF14 (NM_183400) Human Over-expression Lysate

Human

$960
Cat # LS009604
20 µg, 100 µg

RNF14 Overview

Facts about E3 ubiquitin-protein ligase RNF14.

RNF14 3D structure. Source: alphafold

E3 ubiquitin-protein ligase RNF14 (RNF14)

Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which may be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.

Gene Information

Human
Gene Name:	RNF14
Uniprot:	Q9UBS8
Entrez:	9604

Family

Belongs to:

RBR family

Alternative Names

Androgen receptor-associated protein 54; ARA54; ARA54androgen receptor associated protein 54; EC 6.3.2.-; HFB30; HFB30E3 ubiquitin-protein ligase RNF14; ring finger protein 14FLJ26004; RNF14; Triad2 protein; TRIAD2

Molecular Weight

Mass (kDA):
53.837 kDA

Genomic Context


Human

Location:	5q31.3
Sequence:	5; NC_000005.10 (141966807..141990292)

Tissue Specificity

Widely expressed.

Subcellular Localizations

Cytoplasm. Nucleus.

Diseases

Malignant Neoplasms
Malignant Neoplasm Of Prostate
Prostatic Neoplasms
Carcinoma
Neoplasm Metastasis
Neoplasms, Hormone-dependent
Neoplasms
Retinoblastoma
Spinal And Bulbar Muscular Atrophy, X-linked 1
Metastatic Malignant Neoplasm To The Lymph Node
Malignant Neoplasm Of Breast

Colonic Neoplasms
Infertility
Hypokinesia
Azoospermia
Mammary Neoplasms
Chimera Disorder

Interacting Proteins

AR
UBE2D1
UBE2D4

Pathways

Cell Growth
Cell Proliferation
Receptor Transactivation
Reverse Transcription
Cell Cycle
S Phase
Mating
Ovulation

Localization
Spermatogenesis
Chromatin Remodeling

PTMs

Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RNF14

References

PMID: 10320776 by Ueki N., et al. Isolation and characterization of a novel human gene (HFB30) which encodes a protein with a RING finger motif.

PMID: 10085091 by Kang H.-Y., et al. Cloning and characterization of human prostate coactivator ARA54, a novel protein that associates with the androgen receptor.