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Product Info Summary
| SKU: | A01224 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RNF168 Antibody
SKU/Catalog Number
A01224
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RNF168 Antibody (Catalog # A01224). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
RNF168 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RNF168 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01224)
Host
Rabbit
Contents
RNF168 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RNF168 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human RNF168. The immunogen is located within amino acids 440 - 490 of RNF168.
Reactive Species
A01224 is reactive to RNF168 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
65.0 kDa
Background of RNF168
RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01224 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RNF168 antibody can be used for detection of RNF168 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RNF168 in human brain tissue lysate with RNF168 antibody at 1 μg/mL.
Specific Publications For Anti-RNF168 Antibody (A01224)
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