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Product Info Summary
| SKU: | M04190 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RPL10 Mouse Monoclonal Antibody [Clone ID: OTI6B11]
SKU/Catalog Number
M04190
Size
100 µl
Description
Boster Bio RPL10 mouse monoclonal antibody, clone OTI6B11 (formerly 6B11). Catalog# M04190. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-RPL10 Mouse Monoclonal Antibody [Clone ID: OTI6B11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04190)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI6B11
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human RPL10 (NP_006004) produced in E.coli.
Reactive Species
M04190 is reactive to RPL10 in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
24.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04190 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:150
Positive Control
WB: human 293T whole cell, human Hela whole cell, human Jurkat whole cell, human K562 whole cell, rat brain tissue, rat ovary tissue, mouse brain tissue, mouse ovary tissue
Validation Images & Assay Conditions
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Western blot analysis of RPL10 using anti-RPL10 antibody (M04190).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat ovary tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse ovary tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RPL10 antigen affinity purified monoclonal antibody (M04190) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPL10 at approximately 25 kDa. The expected band size for RPL10 is at 25 kDa.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RPL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RPL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
Specific Publications For Anti-RPL10 Mouse Monoclonal Antibody [Clone ID: OTI6B11] (M04190)
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