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Product Info Summary
| SKU: | M02995 |
|---|---|
| Size: | 0.1 ml |
| Reactive Species: | Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-Rt PLSCR1 Ascites Monoclonal Antibody
SKU/Catalog Number
M02995
Size
0.1 ml
Form
Liquid
Description
Boster Bio Anti-Rt PLSCR1 Ascites Monoclonal Antibody (Catalog# M02995). Tested in IP, WB, ICC, Flow Cytometry application(s). This antibody reacts with Rat.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Rt PLSCR1 Ascites Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02995)
Host
Mouse
Contents
Stabilized with 15mM sodium azide.
Clonality
Monoclonal
Clone Number
13A6 [TEC-23]
Isotype
Mouse IgG2a
Immunogen
Pooled lipid raft fraction isolated from RBL-2H3 cells. The antibody 13A6 [TEC-23] binds to an intracellular epitope of rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa protein, accelerating bidirectional movement of plasma membrane phospholipids under conditions of elevated calcium.
Reactive Species
M02995 is reactive to PLSCR1 in Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
36.7 kDa
Background of PLSCR1
PLSCR1 (phospholipid scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02995 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1 μg/ml, positive control: RBL (rat basophilic leukemia) cell line; both reducing and non-reducing conditions.
Flow cytometry: Intracellular staining.
Validation Images & Assay Conditions
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Detection of PLSCR1 in rat basophilic leukemia (RBL) cell line lysate (A) and in PLSCR1 immunoprecipitate from RBL lysate (B). The antibody 13A6 (Tec-23) was used both for immunoprecipitation and immunodetection.
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Immunofluorescence staining of PLSCR1 in rat basophilic leukemia (RBL) cell line using 13A6 (Tec-23) antibody.
Specific Publications For Anti-Rt PLSCR1 Ascites Monoclonal Antibody (M02995)
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