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Product Info Summary
| SKU: | M02995 |
|---|---|
| Size: | 0.1 ml |
| Reactive Species: | Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-Rt PLSCR1 Ascites Monoclonal Antibody
View all Phospholipid Scramblase 1/PLSCR1 Antibodies
SKU/Catalog Number
M02995
Size
0.1 ml
Form
Liquid
Description
Boster Bio Anti-Rt PLSCR1 Ascites Monoclonal Antibody (Catalog# M02995). Tested in IP, WB, ICC, Flow Cytometry application(s). This antibody reacts with Rat.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Rt PLSCR1 Ascites Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02995)
Host
Mouse
Contents
Stabilized with 15mM sodium azide.
Clonality
Monoclonal
Clone Number
13A6 [TEC-23]
Isotype
Mouse IgG2a
Immunogen
Pooled lipid raft fraction isolated from RBL-2H3 cells. The antibody 13A6 [TEC-23] binds to an intracellular epitope of rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa protein, accelerating bidirectional movement of plasma membrane phospholipids under conditions of elevated calcium.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02995 is reactive to PLSCR1 in Rat
Applications
M02995 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
35.049kDa
Background of Phospholipid Scramblase 1/PLSCR1
PLSCR1 (phospholipid scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1 μg/ml, positive control: RBL (rat basophilic leukemia) cell line; both reducing and non-reducing conditions.
Flow cytometry: Intracellular staining.
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of Plscr1 using Anti-Rt PLSCR1 Ascites Monoclonal Antibody (M02995).
Immunofluorescence staining of PLSCR1 in rat basophilic leukemia (RBL) cell line using 13A6 (Tec-23) antibody.
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Figure 1. IP validation of Plscr1 using Anti-Rt PLSCR1 Ascites Monoclonal Antibody (M02995).
Detection of PLSCR1 in rat basophilic leukemia (RBL) cell line lysate (A) and in PLSCR1 immunoprecipitate from RBL lysate (B). The antibody 13A6 (Tec-23) was used both for immunoprecipitation and immunodetection.Protein Target Info & Infographic
Gene/Protein Information For PLSCR1 (Source: Uniprot.org, NCBI)
Gene Name
PLSCR1
Full Name
Phospholipid scramblase 1
Weight
35.049kDa
Superfamily
phospholipid scramblase family
Alternative Names
Erythrocyte phospholipid scramblase; MMTRA1B; MMTRA1BCa(2+)-dependent phospholipid scramblase 1; Phospholipid Scramblase 1; PL scramblase 1; PLSCR1 PLSCR1 MMTRA1B phospholipid scramblase 1 phospholipid scramblase 1|PL scramblase 1|ca(2+)-dependent phospholipid scramblase 1|erythrocyte phospholipid scramblase|mg(2+)-dependent nuclease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLSCR1, check out the PLSCR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLSCR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rt PLSCR1 Ascites Monoclonal Antibody (M02995)
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