Anti-Scramblase 1/PLSCR1 Antibody Picoband™

Product Name

Anti-Scramblase 1/PLSCR1 Antibody Picoband™

View all Phospholipid Scramblase 1/PLSCR1 Antibodies

SKU/Catalog Number

A02995-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Scramblase 1/PLSCR1 Antibody Picoband™ catalog # A02995-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Scramblase 1/PLSCR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02995-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Scramblase 1/PLSCR1 recombinant protein (Position: M1-S54).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02995-2 is reactive to PLSCR1 in Human, Mouse

Applications

A02995-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

35.049kDa

Background of Phospholipid Scramblase 1/PLSCR1

Phospholipid scramblase 1 is a protein that in human is encoded by the PLSCR1 gene. This gene is mapped to 3q24.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PLSCR1 using anti-PLSCR1 antibody (A02995-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLSCR1 antigen affinity purified polyclonal antibody (Catalog # A02995-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLSCR1 at approximately 35 kDa. The expected band size for PLSCR1 is at 35 kDa.

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Figure 2. IHC analysis of PLSCR1 using anti-PLSCR1 antibody (A02995-2).
PLSCR1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PLSCR1 Antibody (A02995-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For PLSCR1 (Source: Uniprot.org, NCBI)

Alternative Names

Erythrocyte phospholipid scramblase; MMTRA1B; MMTRA1BCa(2+)-dependent phospholipid scramblase 1; Phospholipid Scramblase 1; PL scramblase 1; PLSCR1 PLSCR1 MMTRA1B phospholipid scramblase 1 phospholipid scramblase 1|PL scramblase 1|ca(2+)-dependent phospholipid scramblase 1|erythrocyte phospholipid scramblase|mg(2+)-dependent nuclease

For more info on PLSCR1, check out the PLSCR1 Infographic

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In this infographic, you will see the following information for PLSCR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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