Click image to see more details
Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.|
Customers Who Bought This Also Bought
Human EMMPRIN/CD147/BSG ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human EMMPRIN/CD147/BSG ELISA Kit PicoKine™ (96 Tests). Quantitate Human BSG in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EMMPRIN/CD147/BSG ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0751)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: T25-H205
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0751 is reactive to BSG in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
EK0751 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BSG
Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147 (cluster of differentiation 147). It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition (Miyauchi et al., 1991; Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.
|EK0751-CAP||Anti-Human BSG Pre-coated 96-well strip microplate||1|
|EK0751-ST||Human BSG Standard||2 vials, 10 ng/tube|
|EK0751-DA||Human BSG Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Human Emmprin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BSG, Dilution ratio of 1:1, concentration in serum is around 40pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EMMPRIN/CD147/BSG ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of BSG in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For BSG (Source: Uniprot.Org, NCBI)
5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor BSG 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, SLC7A11, TCSF basigin (Ok blood group) basigin|OK blood group antigen|collagenase stimulatory factor|extracellular matrix metalloproteinase inducer|hepatoma-associated antigen|leukocyte activation antigen M6|tumor cell-derived collagenase stimulatory factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on BSG, check out the BSG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BSG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0751 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xie M, Jiao T, Chen Y, Xu C, Li J, Jiang X, Zhang F. Histochem Cell Biol. 2010 May;133(5):585-94. Doi: 10.1007/S00418-010-0697-7. Epub 2010 Apr 6. Emmprin (Basigin/Cd147) Is Involved In The Morphogenesis Of Tooth Germ In Mouse Molars.
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Human EMMPRIN/CD147/BSG ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Human EMMPRIN/CD147/BSG ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question