Anti-S100 beta/S100B Antibody
Rabbit IgG polyclonal antibody for Protein S100-B(S100B) detection. Tested with WB in Human;Mouse;Rat. Cited in 21 publication(s).
Anti-S100 beta/S100B Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-S100 beta/S100B Antibody
See all S100B primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for S100 BETA/S100B detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. S100 BETA/S100B information: Molecular Weight: 10713 MW; Subcellular Localization: Cytoplasm . Nucleus ; Tissue Specificity: Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.|
|Cite This Product||Anti-S100 beta/S100B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1303)|
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|Anti-S100 beta/S100B Antibody may replace the following items: sc 136061|sc 28533|sc 393919|sc 398707|sc 52204|sc 52205|sc 52206|sc 58839|sc 71990|sc 71991|sc 7851|sc 7852|sc 81709.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta(64-78aa DGDGECDFQEFMAFV), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For S100B (Source: Uniprot.org, NCBI)
|NCBI Gene Id||6285|
|Species Of This Entry||Human|
|Protein Name||Protein S100-B|
|Alternative Names||S100B|beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta|
|Post Tranlational Modifications||Phosphorylation; Oxidation; Cleavage; Acetylation; Ubiquitination; Methylation; Nitrosylation; Decarboxylation; Reduction; Carboxylation; Glutathionylation; Citrullination; Dephosphorylation; Biotinylation; Nitration; Glycosylation; Deglycosylation|
|Subcellular Localization||Cytoplasm. Nucleus.|
|Gene Location||21q22.3, on Chromasome 21, gene sequence: NC_000021.9 (46598604..46605243, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For S100B (Source: Uniprot.org, NCBI)
|Protein Function||Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.|
|Tissue Specificity||Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.|
|Interacting Proteins||AGER; MDM2; S100A1; S100A11; S100A2; S100A4; S100A6; S100P; SPG21; TP53|
|Research Areas||Apoptosis; Cell Biology; Cellular Markers; Neuroscience|
|Related Diseases||Brain Injuries; Nervousness; Neoplasms; Traumatic Brain Injury; Cerebrovascular Accident; Alzheimer's Disease; Inflammation; Melanoma; Craniocerebral Trauma; Ischemia; Malignant Neoplasms; Gliosis; Brain Ischemia; Comatose; Hemorrhage; Schizophrenia; Malignant Paraganglionic Neoplasm; Depressive Disorder; Infarction; Neurodegenerative Disorders|
|Related Pathways||Secretion; Pathogenesis; Cell Proliferation; Cell Death; Localization; Inflammatory Response; Aging; Neuroprotection; Regeneration; Cell Cycle; Astrocyte Activation; Cell Growth; Neurogenesis; Cell Adhesion; Protein Phosphorylation; Cell Differentiation; Brain Development; Immune Response; Sensitization; Cell Activation|
|Background||S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery. S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al.(1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.|
|Scientific References||PMID: 2394738 by Allore R.J., et al. Cloning and expression of the human S100 beta gene.|
PMID: 4031854 by Jensen R., et al. Characterization of human brain S100 protein fraction: amino acid sequence of S100 beta.
Our Boster Quality Guarantee for Anti-S100 beta/S100B Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: COLO320 Cell Lysate
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 21
|Liu S, Zhang Y, Zhao Y, Cui H, Cao C, Guo J. Cell Biochem Biophys. 2015 Jan;71(1):401-6. Doi: 10.1007/S12013-014-0212-1. Effects Of Hypothermia On S100B And Glial Fibrillary Acidic Protein In Asphyxia Rats After Cardiopulmonary Resuscitation.||PubMed ID 25209741|
|Chen Q, Zhang Z, Liu J, He Q, Zhou Y, Shao G, Sun X, Cao X, Gong A, Jiang P. Mol Cells. 2015 Mar;38(3):221-8. Doi: 10.14348/Molcells.2015.2170. Epub 2015 Feb 4. A Fibrin Matrix Promotes The Differentiation Of Emscs Isolated From Nasal Respiratory ...||PubMed ID 25666351|
|Tang J, Li Q, Cheng B, Huang C, Chen K. Biomed Rep. 2015 Jul;3(4):499-502. Epub 2015 May 27. Effects Of Diethylstilbestrol On The Proliferation And Tyrosinase Activity Of Cultured Human Melanocytes.||PubMed ID 26171155|
|Xue B, Sun L, Li X, Wang X, Zhang Y, Mu Y, Liang L. Neural Regen Res. 2012 Oct 25;7(30):2340-6. Doi: 10.3969/J.Issn.1673-5374.2012.30.003. Ginsenoside Rb1 Relieves Glucose Fluctuation-Induced Oxidative Stress And Apoptosis In Schwann Cells.||PubMed ID 25538758|
|Wu Y, Xue B, Li X, Liu H. Neural Regen Res. 2012 Nov 25;7(33):2583-91. Doi: 10.3969/J.Issn.1673-5374.2012.33.003. Puerarin Prevents High Glucose-Induced Apoptosis Of Schwann Cells By Inhibiting Oxidative Stress.||PubMed ID 25368634|
|Qu L, Liang X, Gu B, Liu W. Neural Regen Res. 2014 Jun 15;9(12):1195-203. Doi: 10.4103/1673-5374.135328. Quercetin Alleviates High Glucose-Induced Schwann Cell Damage By Autophagy.||PubMed ID 25206782|
|Zhao F, He W, Zhang Y, Tian D, Zhao H, Yu K, Bai J. Neural Regen Res. 2013 Jul 25;8(21):1974-84. Doi: 10.3969/J.Issn.1673-5374.2013.21.006. Electric Stimulation And Decimeter Wave Therapy Improve The Recovery Of Injured Sciatic Nerves.||PubMed ID 25206506|
|Pei H, Su X, Zhao L, Li H, Guo Y, Zhang M, Xin H. Int J Mol Sci. 2012;13(3):2551-62. Doi: 10.3390/Ijms13032551. Epub 2012 Feb 23. Primary Study For The Therapeutic Dose And Time Window Of Picroside Ii In Treating Cerebral Ischemic Injury In Rats.||PubMed ID 22489110|
|Sun D, Li X, Ma M, Liu J, Xu Y, Ye L, Hou H, Wang C, Li X, Jiang Y. Jpn J Clin Oncol. 2015 May;45(5):464-73. Doi: 10.1093/Jjco/Hyv009. Epub 2015 Feb 2. The Predictive Value And Potential Mechanisms Of Mirna-328 And Mirna-378 For Brain Metastases I...||PubMed ID 25646356|
|Jin X, Chen Z, Liu X, Liang B, Zhang H, Zhang Z. Brain Res. 2014 Apr 16;1558:84-9. Doi: 10.1016/J.Brainres.2014.02.032. Epub 2014 Feb 22. The Expression Of Endothelial Barrier Antigen (Eba) And S100B In The Rat Parietal Cortex Following Brain Irra...||PubMed ID 24569094|
|Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo||PubMed ID 28054637|
|Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia||PubMed ID 22684116|
|Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells||PubMed ID 26322102|
|Pro?neurogenic effects of andrographolide on RSC96 Schwann cells in?vitro||PubMed ID 27599453|
|Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-?B signaling in an IL-6-dependent manner||PubMed ID 29178911|
|Graft of a Tissue-Engineered Neural Scaffold Serves as a Promising Strategy to Restore Myelination after Rat Spinal Cord Transection||PubMed ID 24325427|
|Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury||PubMed ID 25682129|
|Dendritic cells and macrophages neurally hard-wired in the lymph node||PubMed ID 26581550|
|A metanephric adenoma of the kidney associated with polycythemia: A case report||PubMed ID 26870216|
|Expression of S100B during the innate immune of corneal epithelium against fungi invasion||PubMed ID 26949634|
|Baicalin promotes the viability of Schwann cells in vitro by regulating neurotrophic factors||PubMed ID 28672960|
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