Anti-S100 beta/S100B Antibody

Boster Bio Anti-S100 beta/S100B Antibody catalog # PA1303. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 21 publication(s).

Product Info Summary

SKU: PA1303
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-S100 beta/S100B Antibody

See all S100B products

SKU/Catalog Number

PA1303

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-S100 beta/S100B Antibody catalog # PA1303. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-S100 beta/S100B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1303)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta (64-78aa DGDGECDFQEFMAFV), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1303 is reactive to S100B in Human, Mouse, Rat

Applications

PA1303 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For S100B (Source: Uniprot.Org, NCBI)

Uniprot ID

P04271

Gene ID

6285

Gene Name

S100B

Full Name

Protein S100-B

Weight

10.713kDa

Superfamily

S-100 family

Alternative Names

beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta S100B NEF, S100, S100-B, S100beta S100 calcium binding protein B protein S100-B|S-100 calcium-binding protein, beta chain|S-100 protein subunit beta|S100 calcium-binding protein, beta (neural)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on S100B, check out the S100B Infographic

S100B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for S100B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1303 has been cited in 21 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Baicalin promotes the viability of Schwann cells in vitro by regulating neurotrophic factors

Expression of S100B during the innate immune of corneal epithelium against fungi invasion

A metanephric adenoma of the kidney associated with polycythemia: A case report

Dendritic cells and macrophages neurally hard-wired in the lymph node

Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury

Graft of a Tissue-Engineered Neural Scaffold Serves as a Promising Strategy to Restore Myelination after Rat Spinal Cord Transection

Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-?B signaling in an IL-6-dependent manner

Pro?neurogenic effects of andrographolide on RSC96 Schwann cells in?vitro

Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells

Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia

Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo

Jin X, Chen Z, Liu X, Liang B, Zhang H, Zhang Z. Brain Res. 2014 Apr 16;1558:84-9. Doi: 10.1016/J.Brainres.2014.02.032. Epub 2014 Feb 22. The Expression Of Endothelial Barrier Antigen (Eba) And S100B In The Rat Parietal Cortex Following Brain Irra...

Sun D, Li X, Ma M, Liu J, Xu Y, Ye L, Hou H, Wang C, Li X, Jiang Y. Jpn J Clin Oncol. 2015 May;45(5):464-73. Doi: 10.1093/Jjco/Hyv009. Epub 2015 Feb 2. The Predictive Value And Potential Mechanisms Of Mirna-328 And Mirna-378 For Brain Metastases I...

Pei H, Su X, Zhao L, Li H, Guo Y, Zhang M, Xin H. Int J Mol Sci. 2012;13(3):2551-62. Doi: 10.3390/Ijms13032551. Epub 2012 Feb 23. Primary Study For The Therapeutic Dose And Time Window Of Picroside Ii In Treating Cerebral Ischemic Injury In Rats.

Zhao F, He W, Zhang Y, Tian D, Zhao H, Yu K, Bai J. Neural Regen Res. 2013 Jul 25;8(21):1974-84. Doi: 10.3969/J.Issn.1673-5374.2013.21.006. Electric Stimulation And Decimeter Wave Therapy Improve The Recovery Of Injured Sciatic Nerves.

Qu L, Liang X, Gu B, Liu W. Neural Regen Res. 2014 Jun 15;9(12):1195-203. Doi: 10.4103/1673-5374.135328. Quercetin Alleviates High Glucose-Induced Schwann Cell Damage By Autophagy.

Wu Y, Xue B, Li X, Liu H. Neural Regen Res. 2012 Nov 25;7(33):2583-91. Doi: 10.3969/J.Issn.1673-5374.2012.33.003. Puerarin Prevents High Glucose-Induced Apoptosis Of Schwann Cells By Inhibiting Oxidative Stress.

Xue B, Sun L, Li X, Wang X, Zhang Y, Mu Y, Liang L. Neural Regen Res. 2012 Oct 25;7(30):2340-6. Doi: 10.3969/J.Issn.1673-5374.2012.30.003. Ginsenoside Rb1 Relieves Glucose Fluctuation-Induced Oxidative Stress And Apoptosis In Schwann Cells.

Tang J, Li Q, Cheng B, Huang C, Chen K. Biomed Rep. 2015 Jul;3(4):499-502. Epub 2015 May 27. Effects Of Diethylstilbestrol On The Proliferation And Tyrosinase Activity Of Cultured Human Melanocytes.

Chen Q, Zhang Z, Liu J, He Q, Zhou Y, Shao G, Sun X, Cao X, Gong A, Jiang P. Mol Cells. 2015 Mar;38(3):221-8. Doi: 10.14348/Molcells.2015.2170. Epub 2015 Feb 4. A Fibrin Matrix Promotes The Differentiation Of Emscs Isolated From Nasal Respiratory ...

Liu S, Zhang Y, Zhao Y, Cui H, Cao C, Guo J. Cell Biochem Biophys. 2015 Jan;71(1):401-6. Doi: 10.1007/S12013-014-0212-1. Effects Of Hypothermia On S100B And Glial Fibrillary Acidic Protein In Asphyxia Rats After Cardiopulmonary Resuscitation.

Have you used Anti-S100 beta/S100B Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-S100 beta/S100B Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-S100 beta/S100B Antibody

Question

I see that the anti-S100 beta/S100B antibody PA1303 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-08

Question

I was wanting to use your anti-S100 beta/S100B antibody for WB for rat skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat skin identification?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

As indicated on the product datasheet, PA1303 anti-S100 beta/S100B antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat skin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-04

Question

I have attached the WB image, lot number and protocol we used for skin using anti-S100 beta/S100B antibody PA1303. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-19

Question

My question regarding product PA1303, anti-S100 beta/S100B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1303 anti-S100 beta/S100B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-10

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-S100 beta/S100B antibody PA1303. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-09

Question

Is this PA1303 anti-S100 beta/S100B antibody reactive to the isotypes of S100B?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

The immunogen of PA1303 anti-S100 beta/S100B antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta(64-78aa DGDGECDFQEFMAFV), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-22

Question

Our lab want to know about to test anti-S100 beta/S100B antibody PA1303 on rat skin for research purposes, then I may be interested in using anti-S100 beta/S100B antibody PA1303 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

The products we sell, including anti-S100 beta/S100B antibody PA1303, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-20

Question

Will anti-S100 beta/S100B antibody PA1303 work for WB with skin?

N. Krishna

Verified customer

Asked: 2019-07-29

Answer

According to the expression profile of skin, S100B is highly expressed in skin. So, it is likely that anti-S100 beta/S100B antibody PA1303 will work for WB with skin.

Boster Scientific Support

Answered: 2019-07-29

Question

We are currently using anti-S100 beta/S100B antibody PA1303 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

The anti-S100 beta/S100B antibody (PA1303) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-12

Question

We have observed staining in rat c1 segment of cervical spinal cord. Are there any suggestions? Is anti-S100 beta/S100B antibody supposed to stain c1 segment of cervical spinal cord positively?

M. Parker

Verified customer

Asked: 2019-06-10

Answer

From literature c1 segment of cervical spinal cord does express S100B. From Uniprot.org, S100B is expressed in c1 segment of cervical spinal cord, skin, among other tissues. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-10

Question

We are interested in using your anti-S100 beta/S100B antibody for traf6 mediated nf-kb activation studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-01-02

Answer

Thanks for your inquiry. This PA1303 anti-S100 beta/S100B antibody is tested on rat brain tissue, tissue lysate, hela cell lysate, smmc cell lysate, jurkat cell lysate, colo320 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-01-02

Question

Is a blocking peptide available for product anti-S100 beta/S100B antibody (PA1303)?

Verified Customer

Verified customer

Asked: 2018-04-09

Answer

We do provide the blocking peptide for product anti-S100 beta/S100B antibody (PA1303). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-04-09

Question

My team were content with the WB result of your anti-S100 beta/S100B antibody. However we have observed positive staining in skin cytoplasm. using this antibody. Is that expected? Could you tell me where is S100B supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-09-04

Answer

From literature, skin does express S100B. Generally S100B expresses in cytoplasm. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-09-04

Question

Is there a BSA free version of anti-S100 beta/S100B antibody PA1303 available?

K. Lewis

Verified customer

Asked: 2017-01-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-S100 beta/S100B antibody PA1303 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-01-23

Question

Does PA1303 anti-S100 beta/S100B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Miller

Verified customer

Asked: 2017-01-13

Answer

You can see on the product datasheet, PA1303 anti-S100 beta/S100B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-01-13

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
PA1303
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.