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Product Info Summary
| SKU: | M12849 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-SCIMP Purified Monoclonal Antibody
SKU/Catalog Number
M12849
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SCIMP Purified Monoclonal Antibody (Catalog# M12849). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-SCIMP Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12849)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
NVL-07
Isotype
Mouse IgG2a
Immunogen
Recombinant intracellular part of human SCIMP. The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M12849 is reactive to SCIMP in Human
Observed Molecular Weight
17-22 kDa
Calculated molecular weight
16.6 kDa
Background of SCIMP
SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12849 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/ml.
Validation Images & Assay Conditions
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Western blotting analysis of human SCIMP using mouse monoclonal antibody NVL-07 on lysates of human SCIMP transfectants under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. SCIMP was detected around 17-22 kDa.
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Separation of human monocytes (red-filled) from SCIMP negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-SCIMP (NVL-07) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).
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Separation of HeLa cells stained using anti-SCIMP (NVL-07) purified antibody (concentration in sample 5 μg/ml, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
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Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-SCIMP (NVL-07) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).
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Flow cytometry analysis (intracellular staining) of SCIMP in a population of HEK-293T-SCIMP transfectants using monoclonal antibody (clone NVL-07, purified).The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.
Specific Publications For Anti-SCIMP Purified Monoclonal Antibody (M12849)
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