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Product Info Summary
| SKU: | A14100 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SH3RF3/Sh3Md4 Antibody
SKU/Catalog Number
A14100
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SH3RF3/Sh3Md4 Antibody (Catalog # A14100). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SH3RF3/Sh3Md4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14100)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human SH3RF3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14100 is reactive to Sh3rf3 in Human, Mouse
Applications
A14100 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
93.13kDa
Background of SH3MD4
Belongs to the SH3RF family.
Hillier L.W., Nature 434:724-731(2005).
Kaerkkaeinen S., EMBO Rep. 7:186-191(2006).
Jikuya H., DNA Res. 10:49-57(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HuvEc cells, using SH3RF3 antibody A14100.
Protein Target Info & Infographic
Gene/Protein Information For Sh3rf3 (Source: Uniprot.org, NCBI)
Gene Name
Sh3rf3
Full Name
E3 ubiquitin-protein ligase SH3RF3
Weight
93.13kDa
Superfamily
SH3RF family
Alternative Names
EC 6.3.2; Plenty Of SH3s 2; Plenty Of SH3s-2; POSH2; SH3 Domain Containing Ring Finger 3; SH3 Domain-Containing RING Finger Protein 3; SH3 Multiple Domains 4; SH3 Multiple Domains Protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sh3rf3, check out the Sh3rf3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sh3rf3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SH3RF3/Sh3Md4 Antibody (A14100)
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No publications found for A14100
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SH3RF3/Sh3Md4 Antibody
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-SH3RF3/Sh3Md4 antibody A14100. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-03
Question
Is there a BSA free version of anti-SH3RF3/Sh3Md4 antibody A14100 available?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SH3RF3/Sh3Md4 antibody A14100 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-31
Question
Can you help my question with product A14100, anti-SH3RF3/Sh3Md4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14100 anti-SH3RF3/Sh3Md4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-SH3RF3/Sh3Md4 antibody A14100 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
The anti-SH3RF3/Sh3Md4 antibody (A14100) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-21
Question
Is this A14100 anti-SH3RF3/Sh3Md4 antibody reactive to the isotypes of SH3RF3?
N. Li
Verified customer
Asked: 2015-12-14
Answer
The immunogen of A14100 anti-SH3RF3/Sh3Md4 antibody is Synthesized peptide derived from C-terminal of human SH3RF3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-12-14
Question
I was wanting to use your anti-SH3RF3/Sh3Md4 antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
M. Roberts
Verified customer
Asked: 2014-06-30
Answer
As indicated on the product datasheet, A14100 anti-SH3RF3/Sh3Md4 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-SH3RF3/Sh3Md4 antibody A14100. Let me know if you need anything else.
M. Parker
Verified customer
Asked: 2014-03-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-03
