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Product Info Summary
| SKU: | A00476-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Skp1 p19 Antibody
SKU/Catalog Number
A00476-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Skp1 p19 Antibody catalog # A00476-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Skp1 p19 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00476-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SKP1A/p19. AA range:41-90
Reactive Species
A00476-1 is reactive to SKP1 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
18.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00476-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Skp1 p19 Polyclonal Antibody
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Western blot analysis of lysates from COS7 cells, using SKP1A/p19 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SKP1A/p19 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Skp1 p19 Antibody (A00476-1)
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4 Customer Q&As for Anti-Skp1 p19 Antibody
Question
I see that the anti-Skp1 p19 antibody A00476-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-05-05
Question
Would anti-Skp1 p19 antibody A00476-1 work on feline WB with skin uterus?
J. Walker
Verified customer
Asked: 2019-10-17
Answer
Our lab technicians have not tested anti-Skp1 p19 antibody A00476-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Skp1 p19 antibody A00476-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline skin uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-17
Question
I was wanting to use your anti-Skp1 p19 antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
As indicated on the product datasheet, A00476-1 anti-Skp1 p19 antibody has been tested for WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-25
Question
Is this A00476-1 anti-Skp1 p19 antibody reactive to the isotypes of SKP1?
A. Anderson
Verified customer
Asked: 2015-07-10
Answer
The immunogen of A00476-1 anti-Skp1 p19 antibody is Synthesized peptide derived from the Internal region of human Skp1 p19. at AA rangle: 10-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-07-10
