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Product Info Summary
| SKU: | M01663 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-GRB10 Antibody Picoband® (monoclonal, 5H7)
SKU/Catalog Number
M01663
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GRB10 Antibody Picoband® (monoclonal, 5H7) catalog # M01663. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRB10 Antibody Picoband® (monoclonal, 5H7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01663)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
5H7
Isotype
Mouse IgG1
Immunogen
E.coli-derived human GRB10 recombinant protein (Position: M1-K251).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01663 is reactive to GRB10 in Human, Monkey, Rat
Observed Molecular Weight
67 kDa
Calculated molecular weight
67.2 kDa
Background of GRB10
GRB10, Growth factor receptor-bound protein 10, also known as insulin receptor-binding protein Grb-IR is a protein that in humans is encoded by the GRB10 gene. The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors (e.g., IGF1R and IGF2R). Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner. Alternatively spliced transcript variants encoding different isoforms have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01663 is guaranteed for Flow Cytometry, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5μg/ml | Human, Monkey, Rat |
| Flow Cytometry (Fixed) | 1-3μg/1x106 cells | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of GRB10 using anti-GRB10 antibody (M01663).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hek293 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat NRK whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GRB10 antigen affinity purified monoclonal antibody (Catalog # M01663) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GRB10 at approximately 67KD. The expected band size for GRB10 is at 67KD.
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Flow Cytometry analysis of THP-1 cells using anti-GRB10 antibody (M01663).
Overlay histogram showing THP-1 cells stained with M01663 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- GRB10 Antibody (M01663, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-GRB10 Antibody Picoband® (monoclonal, 5H7) (M01663)
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