Anti-beta Catenin/CTNNB1 Antibody Picoband™

Boster Bio Anti-beta Catenin/CTNNB1 Antibody Picoband™ catalog # A00004. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 6 publication(s).

Product Info Summary

SKU: A00004
Size: 100ug/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Product Name

Anti-beta Catenin/CTNNB1 Antibody Picoband™

See all beta-Catenin products

SKU/Catalog Number

A00004

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-beta Catenin/CTNNB1 Antibody Picoband™ catalog # A00004. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-beta Catenin/CTNNB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00004)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human beta Catenin recombinant protein (Position: A2-K233).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00004 is reactive to CTNNB1 in Human, Mouse, Rat

Applications

A00004 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/mlbr> Flow Cytometry, 1-3μg/1x106 cellsbr> Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CTNNB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P35222

Gene ID

1499

Gene Name

CTNNB1

Full Name

Catenin beta-1

Weight

85.497kDa

Superfamily

beta-catenin family

Alternative Names

bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923 CTNNB1 CTNNB, EVR7, MRD19, NEDSDV, armadillo catenin beta 1 catenin beta-1|catenin (cadherin-associated protein), beta 1, 88kDa

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CTNNB1, check out the CTNNB1 Infographic

CTNNB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CTNNB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00004 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Piao HY,Guo S,Wang Y,Zhang J.Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.Clin Transl Oncol.2020 Jun 9.doi:10.1007/s12094-020-02412-9.Epub ahead of print.PMID:32519176.
Species: Human
A00004 usage in article: APP:WB, SAMPLE:AGS CELL AND MKN45 CELL, DILUTION:1:200

Dark Aberrant Crypt Foci with activated Wnt pathway are related to tumorigenesis in the colon of AOM-treated rat

Anti-cancer drug 3, 3%u2032-diindolylmethane activates Wnt4 signaling to enhance gastric cancer cell stemness and tumorigenesis

Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition

Li C, Liu J, Zhang Q, Cui K, Ge Q, Wang C, Chen Z. Int J Oncol. 2018 Jun;52(6):1815-1826. doi: 10.3892/ijo.2018.4346. Epub 2018 Mar 29. Upregulation of E-cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder...

Zhang JH, Jiao LY, Li TJ, Zhu YY, Zhou JW, Tian J. J Cancer. 2017 Jun 3;8(9):1598-1608. doi: 10.7150/jca.18744. eCollection 2017. GSK-3β suppresses HCC cell dissociation in vitro by upregulating epithelial junction proteins and inhibiting Wnt/β-ca...

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7 Customer Q&As for Anti-beta Catenin/CTNNB1 Antibody Picoband™

Question

Would anti-beta Catenin/CTNNB1 antibody A00004 work for ICC with colon adenocarcinoma?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

According to the expression profile of colon adenocarcinoma, CTNNB1 is highly expressed in colon adenocarcinoma. So, it is likely that anti-beta Catenin/CTNNB1 antibody A00004 will work for ICC with colon adenocarcinoma.

Boster Scientific Support

Answered: 2020-01-01

Question

Is this A00004 anti-beta Catenin/CTNNB1 antibody reactive to the isotypes of CTNNB1?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

The immunogen of A00004 anti-beta Catenin/CTNNB1 antibody is E. coli-derived human beta Catenin recombinant protein (Position: A2-K233). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-31

Question

I would like to test anti-beta Catenin/CTNNB1 antibody A00004 on rat colon adenocarcinoma for research purposes, then I may be interested in using anti-beta Catenin/CTNNB1 antibody A00004 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

The products we sell, including anti-beta Catenin/CTNNB1 antibody A00004, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-23

Question

Do you have a BSA free version of anti-beta Catenin/CTNNB1 antibody A00004 available?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-beta Catenin/CTNNB1 antibody A00004 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-19

Question

Does A00004 anti-beta Catenin/CTNNB1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

It shows on the product datasheet, A00004 anti-beta Catenin/CTNNB1 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-16

Question

My question regarding product A00004, anti-beta Catenin/CTNNB1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00004 anti-beta Catenin/CTNNB1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-07

Question

Will anti-beta Catenin/CTNNB1 antibody A00004 work on pig WB with adrenal tissue?

Verified Customer

Verified customer

Asked: 2018-04-27

Answer

Our lab technicians have not validated anti-beta Catenin/CTNNB1 antibody A00004 on pig. You can run a BLAST between pig and the immunogen sequence of anti-beta Catenin/CTNNB1 antibody A00004 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig adrenal tissue in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-04-27

Conjugation

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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A00004
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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