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CTNNB1 antibody (A00004) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 15 Publications

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a00004 ctnnb1 primary antibodies fcm testing 10_1

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Flow Cytometry analysis of Hela cells using anti-CTNNB1 antibody (A00004).
Overlay histogram showing Hela cells stained with A00004 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CTNNB1 Antibody (A00004, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Designing a Western blot for CTNNB1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the CTNNB1 Western Blot Guide

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Anti-beta Catenin/CTNNB1 Antibody Picoband®

CTNNB1 antibody

15 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

CTNNB1 antibody was positively detected by WB in:

human MCF-7 whole cell, human A431 whole cell, human RT4 whole cell, human T47D whole cell, human A549 whole cell, human PC-3 whole cell, rat brain tissue, rat C6 whole cell, rat RH35 whole cell, mouse brain tissue, mouse liver tissue, human HEK293- WT whole cell lysates, human HEK293-CTNNB1 KO whole cell lysates

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CTNNB1 antibody was positively detected by IHC in:

human lung cancer tissue, human colon cancer tissue, human mammary cancer tissue, mouse small intestine tissue, rat small intestine tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CTNNB1 antibody was positively detected by ICC/IF in:

U2OS cell

CTNNB1 antibody was positively detected by IF in:

human intestine cancer tissue

CTNNB1 antibody was positively detected by FC in:

Hela cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Rabbit

Predicted

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Zebrafish

Predicted

Product Information

Product Name

Anti-beta Catenin/CTNNB1 Antibody Picoband®
View all CTNNB1 Antibodies

Form

Lyophilized

Uniprot

P35222

Gene ID

1499

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human beta Catenin recombinant protein (Position: A2-K233).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

95 kDa

Calculated Molecular Weight

85.5 kDa

Background of CTNNB1

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Cite This Product

Anti-beta Catenin/CTNNB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00004)

Protocols

Western Blot protocol for CTNNB1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CTNNB1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CTNNB1 antibody

Review Protocol

Immunofluorescence (IF) protocol for CTNNB1 antibody

Review Protocol

Flow Cytometry (FC) protocol for CTNNB1 antibody

Review Protocol

Publications

Exosomes from IL-33-Stimulated Macrophages Regulate Epithelial Barrier Function to Ameliorate TNBS-Induced Colitis in Mice

Published: 2026-07-03

Journal: Cells

Application: IF,WB

Impact Factor: 6.00

Species: Mouse,Human

PMID: 42439691

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Anti-beta Catenin/CTNNB1 Antibody Picoband®

CTNNB1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Exosomes from IL-33-Stimulated Macrophages Regulate Epithelial Barrier Function to Ameliorate TNBS-Induced Colitis in Mice

NsPEFs-enriched ADSCs-EVs alleviate osteoarthritis via RSPO3-mediated dual pro-chondrogenic and pro-M2 macrophage properties

Astragalus polysaccharide regulates the cervical cancer cell cycle and inhibits cisplatin resistance by blocking the Wnt/β-catenin pathway through the PPARD/CDC20 axis

Jiawei Erzhiwan Ameliorates Androgenetic Alopecia by Regulating the SIRT1/JNK/p38 MAPK Pathway

VEGF overexpression in transplanted NSCs promote recovery of neurological function in rats with cerebral ischemia by modulating the Wnt signal transduction pathway

CTHRC1 promotes anaplastic thyroid cancer progression by upregulating the proliferation, migration, and invasion of tumor cells

Lithium chloride promotes neural functional recovery after local cerebral ischemia injury in rats through Wnt signaling pathway activation

Rev-erbα Inhibits Proliferation and Promotes Apoptosis of Preadipocytes through the Agonist GSK4112

MicroRNA-214-3p Targeting Ctnnb1 Promotes 3T3-L1 Preadipocyte Differentiation by Interfering with the Wnt/β-Catenin Signaling Pathway

MDM2 promotes epithelial–mesenchymal transition through activation of Smad2/3 signaling pathway in lung adenocarcinoma

Hypoxia-induced LncRNA PCGEM1 promotes invasion and metastasis of gastric cancer through regulating SNAI1

Probiotic Lactobacillus rhamnosus modulates the gut microbiome composition attenuates preneoplastic colorectal Aberrant crypt foci

Interleukin-27 Inhibits Trophoblast Cell Invasion and Migration by Affecting the Epithelial–Mesenchymal Transition in Preeclampsia

Chronic noise exposure exacerbates AD-like neuropathology in SAMP8 mice in relation to Wnt signaling in the PFC and hippocampus

RRM2 promotes the progression of human glioblastoma

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