Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody
|Applications||IHC-P, IHC-F, WB|
|Product Name||Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 5(SLC2A5) detection. Tested with WB, IHC-P, IHC-F in Mouse;Rat.|
|Cite This Product||Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1737)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of mouse SLC2A5 (232-251aa ALQTLRGWKDVHLEMEEIRK), different from the related rat sequence by two amino acids.|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Anti-SLC2A5 antibody, PA1737, Western blotting
All lanes: Anti SLC2A5 (PA1737) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Mouse Kidney Tissue Lysate at 50ug
Predicted bind size: 55KD
Observed bind size: 55KD
Anti-SLC2A5 antibody, PA1737, IHC(P)
IHC(P): Rat Kidney Tissue
Anti-SLC2A5 antibody, PA1737, IHC(F)
IHC(F): Rat Intestine Tissue
Figure 4. IHC analysis of GLUT5 using anti- GLUT5 antibody (PA1737).
GLUT5 was detected in paraffin-embedded section of mouse ggj3249 tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml rabbit anti- GLUT5 Antibody (PA1737) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Solute carrier family 2, facilitated glucose transporter member 5|
|Alternative Names||Solute carrier family 2, facilitated glucose transporter member 5;Fructose transporter;Glucose transporter type 5, small intestine;GLUT-5;Slc2a5;Glut5;|
|Subcellular Localization||Apical cell membrane ; Multi-pass membrane protein . Membrane ; Multi-pass membrane protein . Localized on the apical membrane of the small intestine and the proximal tubule of the kidney.|
|Molecular Weight||55409 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter (By similarity). .|
|Research Areas||Mouse, Rat
*You can search these to find other products in these research areas.
|Background||GLUT5(Glucose transporter 5), also known as SLC2A5, is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient. In humans the GLUT5 protein is encoded by the SLC2A5 gene.|
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at firstname.lastname@example.org for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact email@example.com
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.