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Product Info Summary
| SKU: | PA2244 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SGLT1/SLC5A1 Antibody Picoband®
SKU/Catalog Number
PA2244
BA3483-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SGLT1/SLC5A1 Antibody catalog # PA2244. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SGLT1/SLC5A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2244)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse SGLT1, different from the related rat sequence by one amino acid, and from the related human sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2244 is reactive to SLC5A1 in Human, Mouse, Rat
Observed Molecular Weight
73 kDa
Calculated molecular weight
73.5 kDa
Background of SLC5A1
Sodium/glucose cotransporter 1 (SLC5A1), also known as NAGT or SGLT1 is a protein that in humans is encoded by the SLC5A1 gene. This gene is mapped to 22q12.3. This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2244 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human HepG2 whole cell, human 293T whole cell, rat kidney tissue, rat NRK whole cell, mouse kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of SLC5A1 using anti-SLC5A1 antibody (PA2244).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat NRK whole cell lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A1 antigen affinity purified polyclonal antibody (Catalog # PA2244) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A1 at approximately 73 kDa. The expected band size for SLC5A1 is at 73 kDa.
Specific Publications For Anti-SGLT1/SLC5A1 Antibody Picoband® (PA2244)
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1 Customer Q&As for Anti-SGLT1/SLC5A1 Antibody Picoband®
Question
We are currently using anti-SGLT1/SLC5A1 antibody PA2244 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
C. Johnson
Verified customer
Asked: 2018-11-26
Answer
The anti-SGLT1/SLC5A1 antibody (PA2244) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-26
