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Product Info Summary
| SKU: | A00090S2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, WB |
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Product info
Product Name
Anti-Smad2/3 (S2) Antibody
SKU/Catalog Number
A00090S2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Smad2/3 (S2) Antibody catalog # A00090S2. Tested in WB,IP,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad2/3 (S2) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00090S2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to Human Smad2/3.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00090S2 is reactive to SMAD2 in Human, Mouse, Rat
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00090S2 is guaranteed for IP, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of Smad2/3 (S2) pAb at 1:1000 dilution Lane1:PC12 whole cell lysate(30ug) Lane2:CT-26 whole cell lysate(30ug) Lane3:Hela whole cell lysate(30ug) Lane4:HEK293T whole cell lysate(30ug) Lane5:EC9706 whole cell lysate(30ug)
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Immunofluorescence analysis of Hela cells using Smad2/3 (S2) pAb at dilution of 1:200.
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Immunoprecipitation of HEK293T cell lysates using Smad2/3 (S2) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using Smad2/3 (S2) pAb.
Specific Publications For Anti-Smad2/3 (S2) Antibody (A00090S2)
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