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SMAD3 antibody (M00059) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 22 Publications

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Anti-Smad3 Rabbit Monoclonal Antibody

SMAD3 antibody

22 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

SMAD3 antibody was positively detected by WB in:

human Jurkat whole cell, human SW620 whole cell, human A431 whole cell, human Hela whole cell, rat lung tissue, rat liver tissue, mouse lung tissue, mouse liver tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

SMAD3 antibody was positively detected by IHC in:

Human lung large cell cancer tissue, Human testis cancer tissue, Human breast cancer tissue, mouse kidney tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Smad3 Rabbit Monoclonal Antibody
View all SMAD3 Antibodies

Form

Liquid

Uniprot

P84022

Gene ID

4088

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

FG-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Smad3

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

50 kDa

Calculated Molecular Weight

48.1 kDa

Cite This Product

Anti-Smad3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00059)

Protocols

Western Blot protocol for SMAD3 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for SMAD3 antibody

Review Protocol

Publications

Tanshinone IIA, salvianic acid A, salvianolic acid B, and hydroxysafflor yellow A reduce cerebral injury via TGF-β1/Smad3 pathway

Published: 2026-05-06

Journal: NEUROSCIENCE LETTERS

Application: IHC,WB

Impact Factor: 2.20

Species: Rat

PMID: 42102961

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Free Secondary Antibody

Anti-Smad3 Rabbit Monoclonal Antibody

SMAD3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Tanshinone IIA, salvianic acid A, salvianolic acid B, and hydroxysafflor yellow A reduce cerebral injury via TGF-β1/Smad3 pathway

The Regulatory Mechanism of MicroRNA-144-3p on Damage to Endometrial Epithelial Cells Exposed to Copper Ions In Vitro

Comparative study on the efficacy of Panax japonicus and its stem-leaf saponins in alleviating renal fibrosis and vascular calcification in mice with chronic kidney disease

Comparison of Anti-Renal Fibrosis Activity of Eucommiae cortex Extract and Its Microbial Fermentation Products

Exosomal miRNA reprogramming in pyroptotic macrophage drives silica-induced fibroblast-to-myofibroblast transition and pulmonary fibrosis

A platform of functional studies of ESCC-associated gene mutations identifies the roles of TGFBR2 in ESCC progression and metastasis

Eucommiae cortex extract alleviates renal fibrosis in CKD mice induced by adenine through the TGF-β1/Smad signaling pathway

Engineering Macrophage-Derived Exosome to Deliver Pirfenidone: A Novel Approach to Combat Silicotic Pulmonary Fibrosis

GGTLC1 knockdown inhibits the progression of endometrial cancer by regulating the TGF-β/Smad signaling pathway

Riboflavin protects against pancreatic cancer metastasis by targeting TGF-β receptor 1

TSLP Induces Epithelial–Mesenchymal Transition in Nasal Epithelial Cells From Allergic Rhinitis Patients Through TGF-β1/Smad2/3 Signaling

Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells

Differential expression of microRNAs in human endometrium after implantation of an intrauterine contraceptive device containing copper

Enhanced transforming growth factor-beta signaling and fibrosis in the pectoralis major muscle of broiler chickens affected by wooden breast myopathy

Mechanistic studies on the role of TGF-β1 in angiogenesis through EndMT

Semaphorin 7A promotes endothelial to mesenchymal transition through ATF3 mediated TGF-β2/Smad signaling

Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF-β1/Smads Signaling Pathway

Reciprocal enhancement of thrombosis by endothelial-to-mesenchymal transition induced by iliac vein compression

Sodium humate accelerates cutaneous wound healing by activating TGF-β/Smads signaling pathway in rats

Molecular responses of radiation-induced liver damage in rats

Dexamethasone attenuates bleomycin-induced lung fibrosis in mice through TGF-β, Smad3 and JAK-STAT pathway

Pathogenesis pathways of idiopathic pulmonary fibrosis in bleomycin-induced lung injury model in mice

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