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Rat TGF Beta 1 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Rat TGF beta 1. 96wells/kit, with removable strips. Cited in 35 publication(s).
Rat TGF Beta 1 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0514 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Rat |
| Sample Type: | cell culture supernates, serum, plasma(EDTA) and urine. |
| Sample Volume: | 100μl per well |
| Sensitivity: | <1pg/ml |
| Assay Range: | 15.6pg/ml-1000pg/ml |
Overview
| Product Name | Rat TGF Beta 1 ELISA Kit PicoKine™
See all Tgfb1 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0514 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Rat Tgf Beta 1/TGFB1 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(EDTA) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Tgf Beta 1/TGFB1 tissue specificity: Abundant in the bone matrix. . |
| Cite This Product | Rat TGF Beta 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0514) |
Product Specs
| Reactivity/Species | Rat |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum, plasma(EDTA) and urine. |
| Sensitivity | <1pg/ml |
| Assay Range | 15.6pg/ml-1000pg/ml |
| Immunogen | Expression system for standard: CHO; Immunogen sequence: A279-S390 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is cross-reactivity with TGFbeta2, TGFbeta3, TGFbeta5<1%. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P17246 |
|---|---|
| Gene Name | Tgfb1 |
| Protein Name | Transforming growth factor beta-1 |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix . |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity). . |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Rat TGF Beta 1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0514-CAP | 96-well plate precoated with anti-Rat Tgfb1 antibody | 1 |
| EK0514-ST | lyophilized recombinant Rat Tgfb1 standard | 10ng/tube |
| EK0514-DA | biotinylated anti-Rat Tgfb1 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0514 Rat TGF Beta 1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.044 | 0.184 | 0.243 | 0.419 | 0.819 | 1.163 | 1.172 | 2.122 |
We measured random samples of Rat TGF Beta 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 27 | 161 | 431 | 28 | 156 | 422 |
| Standard deviation | 1.59 | 9.82 | 23.7 | 1.73 | 9.98 | 26.16 |
| CV(%) | 5.5% | 6.1% | 5.5% | 6.2% | 6.4% | 6.2% |
We measured the performance consistency of Rat TGF Beta 1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 27 | 31 | 29 | 32 | 29 | 1.92 | 6.6% |
| Sample 2 | 161 | 143 | 162 | 145 | 152 | 8.78 | 5.7% |
| Sample 3 | 431 | 413 | 424 | 455 | 430 | 15.4 | 3.5% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 35
| Gao L, Huang W, Li J. Mol Cell Biochem. 2013 Oct;382(1-2):185-91. Doi: 10.1007/S11010-013-1733-4. Epub 2013 Jun 26. Nox1 Abet Mesangial Fibrogenesis Via Inos Induction In Diabetes. | PubMed ID 23801050 |
| Huang Xq, Zhang Xy, Wang Xr, Yu Sy, Fang Sh, Lu Yb, Zhang Wp, Wei Eq. J Neuroinflammation. 2012 Jun 26;9:145. Doi: 10.1186/1742-2094-9-145. Transforming Growth Factor ??1-Induced Astrocyte Migration Is Mediated In Part By Activating 5-Lipoxygenase... | PubMed ID 22734808 |
| Sharifi N, Amani R, Hajiani E, Cheraghian B. Endocrine. 2014 Sep;47(1):70-80. Doi: 10.1007/S12020-014-0336-5. Epub 2014 Jun 27. Does Vitamin D Improve Liver Enzymes, Oxidative Stress, And Inflammatory Biomarkers In Adults With Non-Alcoholic Fatty ... | PubMed ID 24968737 |
| Hou Sx, Zhu Wj, Pang Mq, Jeffry J, Zhou Ll. Food Chem Toxicol. 2014 Feb;64:57-64. Doi: 10.1016/J.Fct.2013.11.022. Epub 2013 Nov 26. Protective Effect Of Iridoid Glycosides From Paederia Scandens (Lour.) Merrill (Rubiaceae) On Uric Acid Nephropathy... | PubMed ID 24287205 |
| Liu Zh, Fan W, Chen Rc. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3441-50. Ecollection 2015. 3,4-Dihydroxyphenylethanol Suppresses Irradiation-Induced Pulmonary Fibrosis In Adult Rats. | PubMed ID 26097528 |
| You H, Wei L, Sun Wl, Wang L, Yang Zl, Liu Y, Zheng K, Wang Y, Zhang Wj. Int J Mol Med. 2014 Jul;34(1):92-102. Doi: 10.3892/Ijmm.2014.1745. Epub 2014 Apr 16. The Green Tea Extract Epigallocatechin-3-Gallate Inhibits Irradiation-Induced Pulmonary F... | PubMed ID 24736877 |
| Wenbin Z, Guojun G. West Indian Med J. 2014 Jan;63(1):20-5. Doi: 10.7727/Wimj.2014.008. Epub 2014 Apr 8. Resveratrol Ameliorates Diabetes-Induced Renal Damage Through Regulating The Expression Of Tgf-??1, Collagen Iv And Th17/Treg-Related Cytokine... | PubMed ID 25303188 |
| Luo, H., Liu, B., Zhao, L., He, J., Li, T., Zha, L.,..., & Yu, Z. (2017). Galectin-3 mediates pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension. Journal of the American Society of Hypertension. Advance online publica... | PubMed ID 28826890 |
| Zhang S, Li J, Li Y, Liu Y, Guo H, Xu X. Front Physiol. 2017 Apr 27;8:249. doi: 10.3389/fphys.2017.00249. eCollection 2017. Nitric Oxide Synthase Activity Correlates with OGG1 in Ozone-Induced Lung Injury Animal Models | PubMed ID 28496412 |
| Sarandy MM, Miranda LL, Alto | PubMed ID 29649320 |
| Santagata S, Napolitano M, D'Alterio C, Desicato S, Maro SD, Marinelli L, Fragale A, Buoncervello M, Persico F, Gabriele L, Novellino E, Longo N, Pignata S, Perdon | PubMed ID 29100374 |
| Effect of Oxymatrine on the TGFbeta-Smad signaling pathway in rats with CCl4-induced hepatic fibrosis | PubMed ID 2701534 |
| Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation | PubMed ID 2761551 |
| The Ayurvedic Medicine | PubMed ID 19706694 |
| Ganoderma lucidum | PubMed ID 22019957 |
| Over-production of nitric oxide by oxidative stress-induced activation of the TGF-?1/PI3K/Akt pathway in mesangial cells cultured in high glucose | PubMed ID 23524565 |
| Correlation between apoptosis and TGF-?1 expression in the mucosal epithelium of rat small intestine in a cold stress state | PubMed ID 23737898 |
| Evening primrose oil ameliorates platelet aggregation and improves cardiac recovery in myocardial-infarct hypercholesterolemic rats | PubMed ID 24665356 |
| Fluorofenidone Offers Improved Renoprotection at Early Interventions during the Course of Diabetic Nephropathy in?db/db?Mice via Multiple Pathways | PubMed ID 25347392 |
| LSKL peptide alleviates subarachnoid fibrosis and hydrocephalus by inhibiting TSP1-mediated TGF-?1 signaling activity following subarachnoid hemorrhage in rats | PubMed ID 27698755 |
| A New Oleanolic Acid Derivative against CCl4-Induced Hepatic Fibrosis in Rats | PubMed ID 28272302 |
| Bacillus Calmette-Guerin alleviates airway inflammation and remodeling by preventing TGF-?1 induced epithelial?mesenchymal transition | PubMed ID 28441064 |
| Efficacies of rosiglitazone and retinoin on bleomycin-induced pulmonary fibrosis in rats | PubMed ID 28672974 |
| Danggui buxue tang suppresses high glucose-induced proliferation and extracellular matrix accumulation of mesangial cells via inhibiting lncRNA PVT1 | PubMed ID 28861164 |
| Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes | PubMed ID 29057272 |
| Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa ?-related pathway in rats | PubMed ID 29928110 |
| Benefical Therapeutic Effect of Chinese Herbal Xinji'erkang Formula on Hypertension-Induced Renal Injury in the 2-Kidney-1-Clip Hypertensive Rats | PubMed ID 25395699 |
| Alterations of serum cytokine levels and their relation with inflammatory markers in candidemia | PubMed ID 25627661 |
| The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells | PubMed ID 30010837 |
| Inhibitive effect of cordyceps sinensis on experimental hepatic fibrosis and its possible mechanism | PubMed ID 12632512 |
| Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo | PubMed ID 22939915 |
| The effects of Panax notoginseng saponins on the cytokines and peritoneal function in rats with peritoneal fibrosis | PubMed ID 26371362 |
| Effect of Oxymatrine on the TGFbeta-Smad signaling pathway in rats with CCl4-induced hepatic fibrosis | PubMed ID 18395914 |
| Induced pluripotent stem cells inhibit bleomycin-induced pulmonary fibrosis in mice through suppressing TGF-%u03B21/Smad-mediated epithelial to mesenchymal %u2026 | PubMed ID 27895584 |
| Rosuvastatin promotes angiogenesis and reverses isoproterenol-induced acute myocardial infarction in rats: role of iNOS and VEGF | PubMed ID 22732653 |
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Questions and answers from customers related to EK0514 Rat TGF Beta 1 ELISA Kit PicoKine™
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Q: What are some alternative names that could be used to describe this product?
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A: Some common names include but are not limited to rat tgfb elisa kit, rat tgfb1 elisa kit, rat tgfbeta elisa kit, rat tgf-beta-1 elisa kit
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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