|Pack Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(EDTA) and urine.|
Data & Images
|Product Name||Rat TGF Beta 1 PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Rat TGF beta 1. 96wells/kit, with removable strips.|
|Cite This Product||Rat TGF Beta 1 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0514)|
|Predicted Species||Bovine, Canine, Monkey, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: CHO; Immunogen sequence: A279-S390|
|Cross Reactivity||There is cross-reactivity with TGFbeta2, TGFbeta3, TGFbeta5<1%.|
|Pack Size||96wells/kit, with removable strips.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, serum, plasma(EDTA) and urine.
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Activating Reagent||TGF beta 1 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGF beta 1.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H2O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml.
|Storage||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|EK0514-CAP||96-well plate precoated with anti-Rat Tgfb1 antibody||1|
|EK0514-ST||lyophilized recombinant Rat Tgfb1 standard||10ng/tubex2|
|EK0514-DA||biotinylated anti-Rat Tgfb1 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
Washing buffer Preparation: Disolve AR0030-E to 1000ml distilled water and adjust pH to 7.2~7.6. Finally, adjust the total volume to 1L.
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Transforming growth factor beta-1|
|Molecular Weight||44329 MW|
|Protein Function||Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity). .|
|Tissue Specificity||Abundant in the bone matrix. .|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix .|
|Alternative Names||Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;Tgfb1;|
|Research Areas|||cardiovascular|angiogenesis|growth factors|tgf| signal transduction|growth factors/hormones| stem cells|signaling pathways|tgf beta|secreted| cancer|invasion/microenvironment|angiogenic growth factors| developmental biology|organogenesis|angiogenesis and vasculogenesis|cancer metabolism|response to hypoxia| metabolism|pathways and processes|cofactors, vitamins / minerals|co-factors|metabolism processes|types of disease||
Background for Transforming growth factor beta-1
Rat TGF Beta 1 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Typical Data Obtained from Rat TGF Beta 1 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-20min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
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