Rat TGF Beta 1 ELISA Kit PicoKine™

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The Rat TGF Beta 1 ELISA Kit PicoKine will detect both active and inactive form of TGF Beta 1. However it will not detect the LAP complex.

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EK0514 detects active TGFb1.

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EK0514 detects active TGFb1.

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The standard for EK0514 Rat TGF Beta 1 ELISA Kit PicoKine should be reconstituted in 400ul to a concentration of 400pg/ml.

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EK0514 is able to detect active TGF beta 1 in activated human and mouse samples. And it has cross reactivity with

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A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

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A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

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A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

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A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGFB1 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TGFB1 that can be declared/interpreted as positive by the above standard is 1pg/ml.

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A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGFB1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

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A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TGFB1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

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A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

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A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

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10% FBS or phenol red in complete cell culture media affects assay using the Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514).

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Yes, the Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514) is able to recognize the mouse version. We haven't got any kit that could recognize both rat and mouse.

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Unfortunately, we cannot disclose the recipe of the color developing reagent (TMB) for the Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514), but we can provide a free bottle to the customer.

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The recommended dilution for rat serum samples using the Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514) is 1:100.

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For the Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514), add 100ul of diluted sample into each well. It is unnecessary to quantify the amount of total protein.

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The Rat TGF Beta 1 ELISA Kit PicoKine EK0514 has not been tested with plasma samples with heparin. We cannot guarantee that it would work. It is suggested to run pilot tests to see if the kits would work on plasma samples with heparin if you would like to try.