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Product Info Summary
| SKU: | A00561 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TIMP1 Antibody Picoband®
SKU/Catalog Number
A00561
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TIMP1 Antibody Picoband® catalog # A00561. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIMP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00561)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TIMP1 recombinant protein (Position: C24-A207).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00561 is reactive to TIMP1 in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
23.2 kDa
Background of TIMP1
TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. TIMP was found to be located about 22 cM proximal to OTC (300461).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00561 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human HL-60 whole cell, human HT29 whole cell, human SKOV3 whole cell, human SiHa whole cell, human U2OS whole cell, human PC-3 whole cell, human THP-1 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TIMP1 using anti-TIMP1 antibody (A00561).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 1: human HL-60 whole cell lysates,
Lane 2: human HT29 whole cell lysates,
Lane 3: human SKOV3 whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP1 antigen affinity purified polyclonal antibody (A00561) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIMP1 at approximately 23 kDa. The expected band size for TIMP1 is at 23 kDa.
Specific Publications For Anti-TIMP1 Antibody Picoband® (A00561)
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